CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018058
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Putative methyltransferase NSUN6 
Protein Synonyms/Alias
 NOL1/NOP2/Sun and PUA domain-containing protein 1; NOL1/NOP2/Sun domain family member 6 
Gene Name
 NSUN6 
Gene Synonyms/Alias
 NOPD1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
6**MSIFPKISLRPEVubiquitination[1]
38GKQEAERKFETLLKHubiquitination[1]
68LASVQHVKNLLLDELubiquitination[1]
109GPRKNIKKQQCEAIVubiquitination[1]
155ISVYSDIKGKCKKGAubiquitination[1]
169AKEFDGTKVFLGNGIubiquitination[1]
182GISELSRKEIFSGLPubiquitination[1]
192FSGLPELKGMGIRMTubiquitination[1]
248LCAAPGGKTTHIAALubiquitination[1]
271ALDKIFNKVEKIKQNubiquitination[1]
296AFCFDGTKAVKLDMVubiquitination[1]
299FDGTKAVKLDMVEDTubiquitination[1]
342MACTWSVKEVASYQPubiquitination[1]
419GLSCEQLKQLQRFDPacetylation[2, 3, 4]
419GLSCEQLKQLQRFDPubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 May have S-adenosyl-L-methionine-dependent methyl- transferase activity (Potential). 
Sequence Annotation
 DOMAIN 111 203 PUA.
 REGION 242 248 S-adenosyl-L-methionine binding (By
 ACT_SITE 373 373 Nucleophile (Potential).
 BINDING 266 266 S-adenosyl-L-methionine (By similarity).
 BINDING 293 293 S-adenosyl-L-methionine (By similarity).
 BINDING 323 323 S-adenosyl-L-methionine (By similarity).
 MOD_RES 419 419 N6-acetyllysine.  
Keyword
 Acetylation; Complete proteome; Methyltransferase; Reference proteome; S-adenosyl-L-methionine; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 469 AA 
Protein Sequence
MSIFPKISLR PEVENYLKEG FMNKEIVTAL GKQEAERKFE TLLKHLSHPP SFTTVRVNTH 60
LASVQHVKNL LLDELQKQFN GLSVPILQHP DLQDVLLIPV IGPRKNIKKQ QCEAIVGAQC 120
GNAVLRGAHV YAPGIVSASQ FMKAGDVISV YSDIKGKCKK GAKEFDGTKV FLGNGISELS 180
RKEIFSGLPE LKGMGIRMTE PVYLSPSFDS VLPRYLFLQN LPSALVSHVL NPQPGEKILD 240
LCAAPGGKTT HIAALMHDQG EVIALDKIFN KVEKIKQNAL LLGLNSIRAF CFDGTKAVKL 300
DMVEDTEGEP PFLPESFDRI LLDAPCSGMG QRPNMACTWS VKEVASYQPL QRKLFTAAVQ 360
LLKPEGVLVY STCTITLAEN EEQVAWALTK FPCLQLQPQE PQIGGEGMRG AGLSCEQLKQ 420
LQRFDPSAVP LPDTDMDSLR EARREDMLRL ANKDSIGFFI AKFVKCKST 469 
Gene Ontology
 GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
 GO:0003723; F:RNA binding; IEA:InterPro. 
Interpro
 IPR001678; Fmu/NOL1/Nop2p.
 IPR018314; Fmu/NOL1/Nop2p_CS.
 IPR002478; PUA.
 IPR015947; PUA-like_domain.
 IPR023267; RCMT. 
Pfam
 PF01189; Nol1_Nop2_Fmu 
SMART
  
PROSITE
 PS01153; NOL1_NOP2_SUN
 PS50890; PUA 
PRINTS
 PR02008; RCMTFAMILY.