CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006800
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transgelin 
Protein Synonyms/Alias
 Actin-associated protein p27; Smooth muscle protein 22-alpha; SM22-alpha 
Gene Name
 Tagln 
Gene Synonyms/Alias
 Sm22; Sm22a 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
17MSREVQSKIEKKYDEacetylation[1]
20EVQSKIEKKYDEELEacetylation[1, 2]
21VQSKIEKKYDEELEEacetylation[2, 3]
75SLYPEGSKPVKVPENacetylation[1, 2]
89NPPSMVFKQMEQVAQacetylation[1, 2]
99EQVAQFLKAAEDYGVacetylation[2]
99EQVAQFLKAAEDYGVubiquitination[4]
121TVDLYEGKDMAAVQRacetylation[1]
154GDPNWFMKKAQEHKRacetylation[1, 2]
172DSQLQEGKHVIGLQMacetylation[1, 2, 5]
Reference
 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [5] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337
Functional Description
 Actin cross-linking/gelling protein (By similarity). 
Sequence Annotation
 DOMAIN 24 137 CH.
 REPEAT 175 200 Calponin-like.
 MOD_RES 2 2 N-acetylalanine (Potential).
 MOD_RES 166 166 Phosphoserine (By similarity).  
Keyword
 3D-structure; Acetylation; Complete proteome; Cytoplasm; Muscle protein; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 201 AA 
Protein Sequence
MANKGPSYGM SREVQSKIEK KYDEELEERL VEWIVVQCGP DVGRPDRGRL GFQVWLKNGV 60
ILSKLVNSLY PEGSKPVKVP ENPPSMVFKQ MEQVAQFLKA AEDYGVIKTD MFQTVDLYEG 120
KDMAAVQRTL MALGSLAVTK NDGNYRGDPN WFMKKAQEHK RDFTDSQLQE GKHVIGLQMG 180
SNRGASQAGM TGYGRPRQII S 201 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0007517; P:muscle organ development; IEA:InterPro. 
Interpro
 IPR000557; Calponin_repeat.
 IPR001715; CH-domain.
 IPR003096; SM22_calponin.
 IPR001061; TAGLN. 
Pfam
 PF00402; Calponin
 PF00307; CH 
SMART
 SM00033; CH 
PROSITE
 PS01052; CALPONIN_1
 PS51122; CALPONIN_2
 PS50021; CH 
PRINTS
 PR00888; SM22CALPONIN.