CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021567
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable cysteine--tRNA ligase, mitochondrial 
Protein Synonyms/Alias
 Cysteinyl-tRNA synthetase; CysRS 
Gene Name
 CARS2 
Gene Synonyms/Alias
 OK/SW-cl.10 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
223GEPADSDKRHASDFAubiquitination[1]
329LKNYITIKDFLKTFSacetylation[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
  
Sequence Annotation
 MOTIF 80 90 "HIGH" region.
 MOTIF 317 321 "KMSKS" region.
 METAL 78 78 Zinc (By similarity).
 METAL 257 257 Zinc (By similarity).
 METAL 282 282 Zinc (By similarity).
 METAL 286 286 Zinc (By similarity).
 BINDING 320 320 ATP (By similarity).  
Keyword
 Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Ligase; Metal-binding; Mitochondrion; Nucleotide-binding; Polymorphism; Protein biosynthesis; Reference proteome; Transit peptide; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 564 AA 
Protein Sequence
MLRTTRGPGL GPPLLQAALG LGRAGWHWPA GRAASGGRGR AWLQPTGRET GVQVYNSLTG 60
RKEPLIVAHA EAASWYSCGP TVYDHAHLGH ACSYVRFDII RRILTKVFGC SIVMVMGITD 120
VDDKIIKRAN EMNISPASLA SLYEEDFKQD MAALKVLPPT VYLRVTENIP QIISFIEGII 180
ARGNAYSTAK GNVYFDLKSR GDKYGKLVGV VPGPVGEPAD SDKRHASDFA LWKAAKPQEV 240
FWASPWGPGR PGWHIECSAI ASMVFGSQLD IHSGGIDLAF PHHENEIAQC EVFHQCEQWG 300
NYFLHSGHLH AKGKEEKMSK SLKNYITIKD FLKTFSPDVF RFFCLRSSYR SAIDYSDSAM 360
LQAQQLLLGL GSFLEDARAY MKGQLACGSV REAMLWERLS STKRAVKAAL ADDFDTPRVV 420
DAILGLAHHG NGQLRASLKE PEGPRSPAVF GAIISYFEQF FETVGISLAN QQYVSGDGSE 480
ATLHGVVDEL VRFRQKVRQF ALAMPEATGD ARRQQLLERQ PLLEACDTLR RGLTAHGINI 540
KDRSSTTSTW ELLDQRTKDQ KSAG 564 
Gene Ontology
 GO:0005829; C:cytosol; IBA:RefGenome.
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004817; F:cysteine-tRNA ligase activity; IBA:RefGenome.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0006423; P:cysteinyl-tRNA aminoacylation; IBA:RefGenome. 
Interpro
 IPR015803; Cys-tRNA-ligase.
 IPR024909; Cys-tRNA/MSH_ligase.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR009080; tRNAsynth_1a_anticodon-bd. 
Pfam
 PF01406; tRNA-synt_1e 
SMART
  
PROSITE
 PS00178; AA_TRNA_LIGASE_I 
PRINTS
 PR00983; TRNASYNTHCYS.