UniProt Accession

 FABI_ECOLI; P0AEK4; P29132 

Genbank Protein ID

 M97219; X78733; U00096; AP009048 

Genbank Nucleotide ID

 AAA17755.1; CAA55381.1; AAC74370.1; BAA14841.1 

Protein Name

 Enoyl-[acyl-carrier-protein] reductase [NADH] FabI 

Protein Synonyms/Alias

 ENR; NADH-dependent enoyl-ACP reductase 

Gene Name

 fabI 

Gene Synonyms/Alias

 envM; b1288; JW1281 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
17	LVTGVASKLSIAYGI	acetylation	[1]
43	AFTYQNDKLKGRVEE	acetylation	[1]
45	TYQNDKLKGRVEEFA	acetylation	[1]
114	AVTREGFKIAHDISS	acetylation	[1]
129	YSFVAMAKACRSMLN	acetylation	[1]
163	YNVMGLAKASLEANV	acetylation	[1]
201	TLAASGIKDFRKMLA	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism and in the biotin biosynthesis. 

Sequence Annotation

 NP_BIND 19 20 NAD.
 NP_BIND 64 65 NAD.
 NP_BIND 192 196 NAD.
 ACT_SITE 146 146 Proton acceptor (By similarity).
 ACT_SITE 156 156 Proton acceptor (By similarity).
 BINDING 13 13 NAD; via carbonyl oxygen.
 BINDING 40 40 NAD.
 BINDING 92 92 NAD; via carbonyl oxygen.
 BINDING 95 95 Substrate; via amide nitrogen and
 BINDING 163 163 NAD.  

Keyword

 3D-structure; Antibiotic resistance; Complete proteome; Direct protein sequencing; Fatty acid biosynthesis; Fatty acid metabolism; Lipid biosynthesis; Lipid metabolism; NAD; Oxidoreductase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 262 AA 

Protein Sequence

Gene Ontology

 GO:0016020; C:membrane; IDA:UniProtKB.
 GO:0004318; F:enoyl-[acyl-carrier-protein] reductase (NADH) activity; IDA:UniProtKB.
 GO:0009102; P:biotin biosynthetic process; IMP:UniProtKB.
 GO:0030497; P:fatty acid elongation; IDA:UniProtKB.
 GO:0051289; P:protein homotetramerization; IDA:UniProtKB.
 GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW. 

Interpro

 IPR014358; Enoyl-ACP_Rdtase_NADH.
 IPR002347; Glc/ribitol_DH.
 IPR016040; NAD(P)-bd_dom. 

Pfam

  

SMART

  

PROSITE

  

PRINTS

 PR00081; GDHRDH.