| Tag | Content |
|---|
| CPLM ID | CPLM-007234 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Inositol phosphosphingolipids phospholipase C |
Protein Synonyms/Alias | IPS phospholipase C; IPS-PLC; Neutral sphingomyelinase; N-SMase; nSMase |
Gene Name | ISC1 |
Gene Synonyms/Alias | YER019W |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 117 | LASACASKYPYQRLF | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] |
Functional Description | Responsible for the hydrolysis of the phosphosphingolipids (IPS), inositol phosphorylceramide (IPC), mannosylinositol phosphorylceramide (MIPC), and mannosyldiinositol phosphorylceramide (M(IP)2C). Also active on sphingomyelin, but this activity is probably not physiologically relevant. |
Sequence Annotation | ACT_SITE 334 334 Proton acceptor (By similarity).
METAL 100 100 Magnesium (By similarity). |
Keyword | Complete proteome; Hydrolase; Lipid metabolism; Magnesium; Membrane; Metal-binding; Reference proteome; Sphingolipid metabolism; Transmembrane; Transmembrane helix. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 477 AA |
Protein Sequence | MYNRKDRDVH ERKEDGQSEF EALNGTNAIM SDNSKAYSIK FLTFNTWGLK YVSKHRKERL 60
RAIADKLAGH SMLTPISDEL LPNGGDSNEN EDYDVIALQE IWCVEDWKYL ASACASKYPY 120
QRLFHSGILT GPGLAILSKV PIESTFLYRF PINGRPSAVF RGDWYVGKSI AITVLNTGTR 180
PIAIMNSHMH APYAKQGDAA YLCHRSCQAW DFSRLIKLYR QAGYAVIVVG DLNSRPGSLP 240
HKFLTQEAGL VDSWEQLHGK QDLAVIARLS PLQQLLKGCT TCDSLLNTWR AQRQPDEACR 300
LDYALIDPDF LQTVDAGVRF TERIPHLDCS VSDHFAYSCT LNIVPQGTES RPSTSVKRAK 360
THDRELILQR YSNYETMIEC IHTYLKTAQR QKFFRGLHFW ASILLLIASL VVTTFTANKA 420
GWSSIFWVLF AIAVSISGTI DGAISFLFGR SEIRALIEVE QEVLDAEHHL QTFLSEK 477 |
Gene Ontology | GO:0005783; C:endoplasmic reticulum; IDA:SGD. GO:0031307; C:integral to mitochondrial outer membrane; IDA:SGD. GO:0052712; F:inositol phosphosphingolipid phospholipase activity; IDA:SGD. GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. GO:0046513; P:ceramide biosynthetic process; IMP:SGD. GO:0009651; P:response to salt stress; IMP:SGD. GO:0030149; P:sphingolipid catabolic process; IDA:SGD. |
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Pfam | |
SMART | |
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PRINTS | |