UniProt Accession

 SRSF1_HUMAN; Q07955; B2R6Z7; D3DTZ3; Q13809 

Genbank Protein ID

 M72709; M72709; M69040; AB062124; AK312781; CH471109; CH471109; BC010264; BC033785 

Genbank Nucleotide ID

 AAA35565.1; AAA35564.1; AAA03476.1; BAB93456.1; BAG35644.1; EAW94485.1; EAW94486.1; AAH10264.1; AAH33785.1 

Protein Name

 Serine/arginine-rich splicing factor 1 

Protein Synonyms/Alias

 Alternative-splicing factor 1; ASF-1; Splicing factor, arginine/serine-rich 1; pre-mRNA-splicing factor SF2, P33 subunit 

Gene Name

 SRSF1 

Gene Synonyms/Alias

 ASF; SF2; SF2P33; SFRS1; OK/SW-cl.3 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
30	LPPDIRTKDIEDVFY	ubiquitination	[1, 2]
38	DIEDVFYKYGAIRDI	ubiquitination	[1]
138	SGSWQDLKDHMREAG	ubiquitination	[1]

Reference

 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'- CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. 

Sequence Annotation

 DOMAIN 16 91 RRM 1.
 DOMAIN 121 195 RRM 2.
 REGION 198 247 Interacts with SAFB1.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 2 2 Phosphoserine.
 MOD_RES 38 38 N6-acetyllysine.
 MOD_RES 179 179 N6-acetyllysine.
 MOD_RES 199 199 Phosphoserine.
 MOD_RES 201 201 Phosphoserine (By similarity).
 MOD_RES 205 205 Phosphoserine.
 MOD_RES 231 231 Phosphoserine.
 MOD_RES 234 234 Phosphoserine.
 MOD_RES 238 238 Phosphoserine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; RNA-binding; Spliceosome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 248 AA 

Protein Sequence

Gene Ontology

 GO:0071013; C:catalytic step 2 spliceosome; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016607; C:nuclear speck; IDA:MGI.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IDA:UniProtKB.
 GO:0060048; P:cardiac muscle contraction; IEA:Compara.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0031124; P:mRNA 3'-end processing; TAS:Reactome.
 GO:0000395; P:mRNA 5'-splice site recognition; IDA:UniProtKB.
 GO:0006406; P:mRNA export from nucleus; TAS:Reactome.
 GO:0006369; P:termination of RNA polymerase II transcription; TAS:Reactome. 

Interpro

 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 

Pfam

 PF00076; RRM_1 

SMART

 SM00360; RRM 

PROSITE

 PS50102; RRM 

PRINTS