CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012365
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Clathrin interactor 1 
Protein Synonyms/Alias
 Clathrin-interacting protein localized in the trans-Golgi region; Clint; Enthoprotin; Epsin-4; Epsin-related protein; EpsinR 
Gene Name
 CLINT1 
Gene Synonyms/Alias
 ENTH; EPN4; EPNR; KIAA0171 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
158KAKKNKDKYVGVSSDubiquitination[1]
183YDPEPKSKWDEEWDKubiquitination[1]
200SAFPFSDKLGELSDKubiquitination[1]
218TIDDTISKFRRKDREubiquitination[1]
471NVSTVLQKPNPLYNQubiquitination[1, 2]
485QNTDMVQKSVSKTLPubiquitination[1, 2]
Reference
 [1] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Binds to membranes enriched in phosphatidylinositol 4,5- bisphosphate (PtdIns(4,5)P2). May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. Stimulates clathrin assembly. 
Sequence Annotation
 DOMAIN 16 149 ENTH.
 BINDING 29 29 Phosphatidylinositol lipid headgroup (By
 BINDING 67 67 Phosphatidylinositol lipid headgroup (By
 MOD_RES 163 163 Phosphoserine (By similarity).
 MOD_RES 227 227 Phosphoserine.
 MOD_RES 232 232 Phosphoserine (By similarity).
 MOD_RES 234 234 Phosphoserine (By similarity).
 MOD_RES 245 245 Phosphoserine.
 MOD_RES 299 299 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Direct protein sequencing; Endocytosis; Lipid-binding; Membrane; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 625 AA 
Protein Sequence
MLNMWKVREL VDKATNVVMN YSEIESKVRE ATNDDPWGPS GQLMGEIAKA TFMYEQFPEL 60
MNMLWSRMLK DNKKNWRRVY KSLLLLAYLI RNGSERVVTS AREHIYDLRS LENYHFVDEH 120
GKDQGINIRQ KVKELVEFAQ DDDRLREERK KAKKNKDKYV GVSSDSVGGF RYSERYDPEP 180
KSKWDEEWDK NKSAFPFSDK LGELSDKIGS TIDDTISKFR RKDREDSPER CSDSDEEKKA 240
RRGRSPKGEF KDEEETVTTK HIHITQATET TTTRHKRTAN PSKTIDLGAA AHYTGDKASP 300
DQNASTHTPQ SSVKTSVPSS KSSGDLVDLF DGTSQSTGGS ADLFGGFADF GSAAASGSFP 360
SQVTATSGNG DFGDWSAFNQ APSGPVASSG EFFGSASQPA VELVSGSQSA LGPPPAASNS 420
SDLFDLMGSS QATMTSSQSM NFSMMSTNTV GLGLPMSRSQ PLQNVSTVLQ KPNPLYNQNT 480
DMVQKSVSKT LPSTWSDPSV NISLDNLLPG MQPSKPQQPS LNTMIQQQNM QQPMNVMTQS 540
FGAVNLSSPS NMLPVRPQTN ALIGGPMPMS MPNVMTGTMG MAPLGNTPMM NQSMMGMNMN 600
IGMSAAGMGL TGTMGMGMPN IAMTSGTVQP KQDAFANFAN FSK 643 
Gene Ontology
 GO:0030136; C:clathrin-coated vesicle; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005794; C:Golgi apparatus; IDA:UniProtKB.
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
 GO:0016044; P:cellular membrane organization; TAS:Reactome.
 GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
 GO:0006892; P:post-Golgi vesicle-mediated transport; TAS:Reactome. 
Interpro
 IPR008942; ENTH_VHS.
 IPR013809; Epsin-like_N.
 IPR001026; Epsin_dom_N. 
Pfam
 PF01417; ENTH 
SMART
 SM00273; ENTH 
PROSITE
 PS50942; ENTH 
PRINTS