CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018377
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein ELYS 
Protein Synonyms/Alias
 Embryonic large molecule derived from yolk sac; Protein MEL-28; Putative AT-hook-containing transcription factor 1 
Gene Name
 AHCTF1 
Gene Synonyms/Alias
 ELYS; TMBS62; MSTP108 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
72LESVLRGKFAAGKNGubiquitination[1]
77RGKFAAGKNGLACLAubiquitination[1]
125VKEFSWQKRTGLLIGubiquitination[1]
153LGISKVVKAVVLPGRubiquitination[1]
347QLAFGNRKCLASGQIubiquitination[1]
385RGQTSNTKLLGCQSIubiquitination[1, 2]
394LGCQSIEKFRSHGDRubiquitination[1]
539KETLTFLKKSGPSLNubiquitination[1]
540ETLTFLKKSGPSLNEubiquitination[1]
752FERLSRGKWNPDCLMubiquitination[1, 3]
1004ANYVPALKLNQTLKIubiquitination[1]
1010LKLNQTLKINVMNDRubiquitination[1]
1037SILDQYGKILPRVHRubiquitination[1, 3]
1052KLAIERAKPYHLSTSubiquitination[1]
1076KPLSAVPKQVVTGTVubiquitination[2]
1104IGEVWASKEPINSTTubiquitination[1, 2, 4]
1144FFGTPISKASQKISRubiquitination[4]
1176FIQQSSMKSPLYLVSubiquitination[1]
1193LPSSSQLKGSPQAISubiquitination[1, 2]
1216ETPLVVKKAKSLAMSubiquitination[1]
1712ENLLDVIKDTRSKEIubiquitination[4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Required for the assembly of a functional nuclear pore complex (NPC) on the surface of chromosomes as nuclei form at the end of mitosis. May initiate NPC assembly by binding to chromatin and recruiting the Nup107-160 subcomplex of the NPC. Also required for the localization of the Nup107-160 subcomplex of the NPC to the kinetochore during mitosis and for the completion of cytokinesis. 
Sequence Annotation
 DNA_BIND 1971 1983 A.T hook.
 REGION 1 981 Necessary for cytoplasmic localization
 REGION 1149 2266 Necessary for nuclear localization (By
 REGION 1446 1698 Mediates transcriptional activity (By
 MOD_RES 528 528 Phosphoserine.
 MOD_RES 1142 1142 Phosphoserine.
 MOD_RES 1150 1150 Phosphoserine.
 MOD_RES 1155 1155 Phosphoserine.
 MOD_RES 1160 1160 Phosphoserine.
 MOD_RES 1175 1175 Phosphothreonine.
 MOD_RES 1214 1214 Phosphoserine.
 MOD_RES 1222 1222 Phosphoserine.
 MOD_RES 1232 1232 Phosphoserine.
 MOD_RES 1250 1250 Phosphoserine.
 MOD_RES 1283 1283 Phosphoserine.
 MOD_RES 1297 1297 Phosphoserine.
 MOD_RES 1369 1369 Phosphothreonine.
 MOD_RES 1371 1371 Phosphoserine (By similarity).
 MOD_RES 1513 1513 Phosphoserine.
 MOD_RES 1517 1517 Phosphothreonine.
 MOD_RES 1533 1533 Phosphoserine.
 MOD_RES 1541 1541 Phosphoserine.
 MOD_RES 1729 1729 Phosphoserine.
 MOD_RES 1806 1806 Phosphoserine.
 MOD_RES 1808 1808 Phosphothreonine.
 MOD_RES 1878 1878 Phosphoserine.
 MOD_RES 1884 1884 Phosphoserine.
 MOD_RES 1898 1898 Phosphoserine.
 MOD_RES 1944 1944 Phosphoserine.
 MOD_RES 1946 1946 Phosphoserine.
 MOD_RES 1996 1996 Phosphoserine.
 MOD_RES 2043 2043 Phosphoserine.
 MOD_RES 2044 2044 Phosphoserine.
 MOD_RES 2060 2060 Phosphoserine.
 MOD_RES 2089 2089 Phosphoserine.
 MOD_RES 2120 2120 Phosphoserine.
 MOD_RES 2123 2123 Phosphoserine.
 MOD_RES 2154 2154 Phosphoserine.
 MOD_RES 2212 2212 Phosphoserine.
 MOD_RES 2222 2222 Phosphoserine.
 MOD_RES 2226 2226 Phosphoserine.  
Keyword
 Alternative splicing; Cell cycle; Cell division; Centromere; Chromosome; Complete proteome; Cytoplasm; DNA-binding; Kinetochore; mRNA transport; Nuclear pore complex; Nucleus; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Translocation; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2266 AA 
Protein Sequence
MDSLAGLSPR EAAGVLSLSC VGVCSTCAWA WPHGSMRDLR AQVTSGLLPF PEVTLQALGE 60
DEITLESVLR GKFAAGKNGL ACLACGPQLE VVNSITGERL SAYRFSGVNE QPPVVLAVKE 120
FSWQKRTGLL IGLEETEGSV LCLYDLGISK VVKAVVLPGR VTAIEPIINH GGASASTQHL 180
HPSLRWLFGV AAVVTDVGQI LLVDLCLDDL SCNQNEVEAS DLEVLTGIPA EVPHIRESVM 240
RQGRHLCFQL VSPTGTAVST LSYISRTNQL AVGFSDGYLA LWNMKSMKRE YYIQLESGQV 300
PVYAVTFQEP ENDPRNCCYL WAVQSTQDSE GDVLSLHLLQ LAFGNRKCLA SGQILYEGLE 360
YCEERYTLDL TGGMFPLRGQ TSNTKLLGCQ SIEKFRSHGD REEGVNEALS PDTSVSVFTW 420
QVNIYGQGKP SVYLGLFDIN RWYHAQMPDS LRSGEYLHNC SYFALWSLES VVSRTSPHGI 480
LDILVHERSL NRGVPPSYPP PEQFFNPSTY NFDATCLLNS GVVHLTCTGF QKETLTFLKK 540
SGPSLNELIP DGYNRCLVAG LLSPRFVDVQ PSSLSQEEQL EAILSAAIQT SSLGLLTGYI 600
RRWITEEQPN SATNLRFVLE WTWNKVVLTK EEFDRLCVPL FDGSCHFMDP QTIQSIQQCY 660
LLLSNLNIVL SCFASEAREI TERGLIDLSN KFVVSHLICQ YAQVVLWFSH SGLLPEGIDD 720
SVQLSRLCYN YPVIQNYYTS RRQKFERLSR GKWNPDCLMI DGLVSQLGER IEKLWKRDEG 780
GTGKYPPASL HAVLDMYLLD GVTEAAKHSI TIYLLLDIMY SFPNKTDTPI ESFPTVFAIS 840
WGQVKLIQGF WLIDHNDYES GLDLLFHPAT AKPLSWQHSK IIQAFMSQGE HRQALRYIQT 900
MKPTVSSGND VILHLTVLLF NRCMVEAWNF LRQHCNRLNI EELLKHMYEV CQEMGLMEDL 960
LKLPFTDTEQ ECLVKFLQSS ASVQNHEFLL VHHLQRANYV PALKLNQTLK INVMNDRDPR 1020
LRERSLARNS ILDQYGKILP RVHRKLAIER AKPYHLSTSS VFRLVSRPKP LSAVPKQVVT 1080
GTVLTRSVFI NNVLSKIGEV WASKEPINST TPFNSSKIEE PSPIVYSLPA PELPEAFFGT 1140
PISKASQKIS RLLDLVVQPV PRPSQCSEFI QQSSMKSPLY LVSRSLPSSS QLKGSPQAIS 1200
RASELHLLET PLVVKKAKSL AMSVTTSGFS EFTPQSILRS TLRSTPLASP SPSPGRSPQR 1260
LKETRISFVE EDVHPKWIPG AADDSKLEVF TTPKKCAVPV ETEWLKSKDR TTSFFLNSPE 1320
KEHQEMDEGS QSLEKLDVSK GNSSVSITSD ETTLEYQDAP SPEDLEETVF TASKPKSSST 1380
ALTTNVTEQT EKDGDKDVFA SEVTPSDLQK QMGNLEDAET KDLLVAAEAF SELNHLSPVQ 1440
GTEASLCAPS VYEGKIFTQK SKVPVLDEGL TSVETYTPAI RANDNKSMAD VLGDGGNSSL 1500
TISEGPIVSE RRLNQEVALN LKEDHEVEVG VLKESVDLPE EKLPISDSPP DTQEIHVIEQ 1560
EKLEAQDSGE EARNLSFNEL YPSGTLKLQY NFDTIDQQFC DLADNKDTAE CDIAEVDGEL 1620
FVAQSNFTLI LEGEEGEVEP GDFASSDVLP KAANTATEEK LVCSGENDNH GQIANLPSAV 1680
TSDQKSQKVD TLPYVPEPIK VAIAENLLDV IKDTRSKEIT SDTMEQSIHE TIPLVSQNIM 1740
CPTKLVKSAF KTAQETSTMT MNVSQVDDVV SSKTRTRGQR IQNVNVKSAQ QEASADVATP 1800
KMPGQSVRKK TRKAKEISEA SENIYSDVRG LSQNQQIPQN SVTPRRGRRK KEVNQDILEN 1860
TSSVEQELQI TTGRESKRLK SSQLLEPAVE ETTKKEVKVS SVTKRTPRRI KRSVENQESV 1920
EIINDLKVST VTSPSRMIRK LRSTNLDASE NTGNKQDDKS SDKQLRIKHV RRVRGREVSP 1980
SDVREDSNLE SSQLTVQAEF DMSAIPRKRG RPRKINPSED VGSKAVKEER SPKKKEAPSI 2040
RRRSTRNTPA KSENVDVGKP ALGKSILVPN EELSMVMSSK KKLTKKTESQ SQKRSLHSVS 2100
EERTDEMTHK ETNEQEERLL ATASFTKSSR SSRTRSSKAI LLPDLSEPNN EPLFSPASEV 2160
PRKAKAKKIE VPAQLKELVS DLSSQFVISP PALRSRQKNT SNKNKLEDEL KDDAQSVETL 2220
GKPKAKRIRT SKTKQASKNT EKESAWSPPP IEIRLISPLA SPADGVKSKP RKTTEVTGTG 2280
LGRNRKKLSS YPKQILRRKM L 2301 
Gene Ontology
 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016363; C:nuclear matrix; IEA:UniProtKB-SubCell.
 GO:0031965; C:nuclear membrane; IDA:HPA.
 GO:0005643; C:nuclear pore; IEA:UniProtKB-SubCell.
 GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:Compara.
 GO:0000910; P:cytokinesis; IMP:UniProtKB.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome.
 GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
 GO:0051292; P:nuclear pore complex assembly; IMP:UniProtKB.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 
Interpro
 IPR017956; AT_hook_DNA-bd_motif.
 IPR025151; ELYS_dom. 
Pfam
 PF13934; ELYS 
SMART
 SM00384; AT_hook 
PROSITE
  
PRINTS