CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008945
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Microfibrillar-associated protein 1 
Protein Synonyms/Alias
  
Gene Name
 MFAP1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
200DEMEPRLKPVFIRKKacetylation[1]
200DEMEPRLKPVFIRKKubiquitination[2]
230KELEQEAKRMAEERRubiquitination[3]
280EEEYEAWKVRELKRIubiquitination[2]
340GKYKFLQKYYHRGAFacetylation[4]
340GKYKFLQKYYHRGAFubiquitination[2]
357DEDEEVYKRDFSAPTubiquitination[1, 2, 3, 5, 6, 7, 8]
371TLEDHFNKTILPKVMubiquitination[1, 2, 3, 8]
376FNKTILPKVMQVKNFubiquitination[9, 10]
381LPKVMQVKNFGRSGRubiquitination[1, 2, 3]
390FGRSGRTKYTHLVDQubiquitination[2, 3, 8, 9, 10]
415QESAQNTKFFKQKAAubiquitination[3, 8, 9, 10]
Reference
 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [8] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [9] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [10] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
 Component of the elastin-associated microfibrils. 
Sequence Annotation
 MOD_RES 52 52 Phosphoserine.
 MOD_RES 53 53 Phosphoserine.
 MOD_RES 116 116 Phosphoserine.
 MOD_RES 118 118 Phosphoserine.
 MOD_RES 132 132 Phosphoserine.
 MOD_RES 133 133 Phosphoserine.
 MOD_RES 211 211 Phosphothreonine (By similarity).
 MOD_RES 267 267 Phosphothreonine.  
Keyword
 Complete proteome; Extracellular matrix; Phosphoprotein; Reference proteome; Secreted. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 439 AA 
Protein Sequence
MSVPSALMKQ PPIQSTAGAV PVRNEKGEIS MEKVKVKRYV SGKRPDYAPM ESSDEEDEEF 60
QFIKKAKEQE AEPEEQEEDS SSDPRLRRLQ NRISEDVEER LARHRKIVEP EVVGESDSEV 120
EGDAWRMERE DSSEEEEEEI DDEEIERRRG MMRQRAQERK NEEMEVMEVE DEGRSGEESE 180
SESEYEEYTD SEDEMEPRLK PVFIRKKDRV TVQEREAEAL KQKELEQEAK RMAEERRKYT 240
LKIVEEETKK ELEENKRSLA ALDALNTDDE NDEEEYEAWK VRELKRIKRD REDREALEKE 300
KAEIERMRNL TEEERRAELR ANGKVITNKA VKGKYKFLQK YYHRGAFFMD EDEEVYKRDF 360
SAPTLEDHFN KTILPKVMQV KNFGRSGRTK YTHLVDQDTT SFDSAWGQES AQNTKFFKQK 420
AAGVRDVFER PSAKKRKTT 439 
Gene Ontology
 GO:0001527; C:microfibril; IDA:UniProtKB.
 GO:0030198; P:extracellular matrix organization; TAS:Reactome. 
Interpro
 IPR009730; MFAP1_C. 
Pfam
 PF06991; Prp19_bind 
SMART
  
PROSITE
  
PRINTS