UniProt Accession

 NADE_ECOLI; P18843; P78235 

Genbank Protein ID

 M15328; U00096; AP009048 

Genbank Nucleotide ID

 AAA79852.1; AAC74810.1; BAA15529.1 

Protein Name

 NH(3)-dependent NAD(+) synthetase 

Protein Synonyms/Alias

 Nicotinamide adenine dinucleotide synthetase; NADS; Nitrogen regulatory protein 

Gene Name

 nadE 

Gene Synonyms/Alias

 efg; ntrL; b1740; JW1729 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
9	TLQQQIIKALGAKPQ	acetylation	[1, 2]
14	IIKALGAKPQINAEE	acetylation	[2]
207	ACPEHLYKKAPTADL	acetylation	[1, 2]
257	TIENWYLKTEHKRRP	acetylation	[2]
261	WYLKTEHKRRPPITV	acetylation	[2]
274	TVFDDFWKK******	acetylation	[2]

Reference

 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes a key step in NAD biosynthesis, transforming deamido-NAD into NAD by a two-step reaction. 

Sequence Annotation

 NP_BIND 46 53 ATP.
 NP_BIND 170 180 NAD.
 BINDING 33 33 NAD.
 BINDING 82 82 ATP.
 BINDING 88 88 ATP (By similarity).
 BINDING 140 140 NAD.
 BINDING 160 160 ATP.
 BINDING 211 211 ATP (By similarity).
 BINDING 261 261 NAD.  

Keyword

 3D-structure; ATP-binding; Complete proteome; Direct protein sequencing; Ligase; NAD; Nucleotide-binding; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 275 AA 

Protein Sequence

Gene Ontology

 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0003952; F:NAD+ synthase (glutamine-hydrolyzing) activity; IEA:InterPro.
 GO:0008795; F:NAD+ synthase activity; IDA:EcoCyc.
 GO:0034628; P:de novo NAD biosynthetic process from aspartate; IDA:EcoCyc.
 GO:0034355; P:NAD salvage; IDA:EcoCyc.
 GO:0006974; P:response to DNA damage stimulus; IEP:EcoliWiki. 

Interpro

 IPR022310; NAD/GMP_synthase.
 IPR003694; NAD_synthase.
 IPR022926; NH(3)-dep_NAD(+)_synth.
 IPR014729; Rossmann-like_a/b/a_fold. 

Pfam

 PF02540; NAD_synthase 

SMART

  

PROSITE

  

PRINTS