CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031396
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial 
Protein Synonyms/Alias
 cDNA FLJ55007, highly similar to Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial 
Gene Name
 MTHFD2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
30TGIPTLGKNVVVAGRubiquitination[1, 2, 3, 4, 5]
73ISHRYTPKEQLKKHTubiquitination[3]
122VHDPVTAKPKLVGDVacetylation[6]
122VHDPVTAKPKLVGDVubiquitination[1, 3, 4]
124DPVTAKPKLVGDVDFubiquitination[4]
137DFEGVRQKAGYITPVubiquitination[2]
177LEEREVLKSKELGVAubiquitination[7]
179EREVLKSKELGVATNubiquitination[1, 3, 4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Hydrolase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 186 AA 
Protein Sequence
MCLDQYSMLP ATPWGVWEII KRTGIPTLGK NVVVAGRSKN VGMPIAMLLH TDGAHERPGG 60
DATVTISHRY TPKEQLKKHT ILADIVISAA GIPNLITADM IKEGAAVIDV GINRVHDPVT 120
AKPKLVGDVD FEGVRQKAGY ITPVPGGVGP MTVAMLMKNT IIAAKKVLRL EEREVLKSKE 180
LGVATN 186 
Gene Ontology
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
 GO:0004488; F:methylenetetrahydrofolate dehydrogenase (NADP+) activity; IEA:InterPro.
 GO:0009396; P:folic acid-containing compound biosynthetic process; IEA:InterPro.
 GO:0009408; P:response to heat; IEA:Compara. 
Interpro
 IPR016040; NAD(P)-bd_dom.
 IPR000672; THF_DH/CycHdrlase.
 IPR020867; THF_DH/CycHdrlase_CS.
 IPR020631; THF_DH/CycHdrlase_NAD-bd_dom. 
Pfam
 PF02882; THF_DHG_CYH_C 
SMART
  
PROSITE
 PS00767; THF_DHG_CYH_2 
PRINTS
 PR00085; THFDHDRGNASE.