CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004139
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein STE12 
Protein Synonyms/Alias
  
Gene Name
 STE12 
Gene Synonyms/Alias
 YHR084W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
174DALERDLKRESLNQPsumoylation[1]
409SDPTSYMKYDEPVMDsumoylation[1]
682DTNRRSDKNLPDAT*acetylation[2]
Reference
 [1] Pheromone-regulated sumoylation of transcription factors that mediate the invasive to mating developmental switch in yeast.
 Wang Y, Dohlman HG.
 J Biol Chem. 2006 Jan 27;281(4):1964-9. [PMID: 16306045]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Binds to the DNA sequence mediating pheromone induction (called the pheromone response element = PRE) which is found in the upstream control region of several a-, alpha- and haploid- specific genes. Involved in mating of haploids and in pseudohyphae formation in diploids. 
Sequence Annotation
 DNA_BIND 57 167
 MOD_RES 29 29 Phosphothreonine.
 MOD_RES 214 214 Phosphoserine.
 MOD_RES 226 226 Phosphoserine.
 MOD_RES 289 289 Phosphothreonine.
 MOD_RES 400 400 Phosphoserine.  
Keyword
 3D-structure; Activator; Complete proteome; DNA-binding; Nucleus; Pheromone response; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 688 AA 
Protein Sequence
MKVQITNSRT EEILKVQANN ENDEVSKATP GEVEESLRLI GDLKFFLATA PVNWQENQII 60
RRYYLNSGQG FVSCVFWNNL YYITGTDIVK CCLYRMQKFG REVVQKKKFE EGIFSDLRNL 120
KCGIDATLEQ PKSEFLSFLF RNMCLKTQKK QKVFFWFSVA HDKLFADALE RDLKRESLNQ 180
PSTTKPVNEP ALSFSYDSSS DKPLYDQLLQ HLDSRRPSST TKSDNSPPKL ESENFKDNEL 240
VTVTNQPLLG VGLMDDDAPE SPSQINDFIP QKLIIEPNTL ELNGLTEETP HDLPKNTAKG 300
RDEEDFPLDY FPVSVEYPTE ENAFDPFPPQ AFTPAAPSMP ISYDNVNERD SMPVNSLLNR 360
YPYQLSVAPT FPVPPSSSRQ HFMTNRDFYS SNNNKEKLVS PSDPTSYMKY DEPVMDFDES 420
RPNENCTNAK SHNSGQQTKQ HQLYSNNFQQ SYPNGMVPGY YPKMPYNPMG GDPLLDQAFY 480
GADDFFFPPE GCDNNMLYPQ TATSWNVLPP QAMQPAPTYV GRPYTPNYRS TPGSAMFPYM 540
QSSNSMQWNT AVSPYSSRAP STTAKNYPPS TFYSQNINQY PRRRTVGMKS SQGNVPTGNK 600
QSVGKSAKIS KPLHIKTSAY QKQYKINLET KARPSAGDED SAHPDKNKEI SMPTPDSNTL 660
VVQSEEGGAH SLEVDTNRRS DKNLPDAT 688 
Gene Ontology
 GO:0005654; C:nucleoplasm; IDA:SGD.
 GO:0043565; F:sequence-specific DNA binding; IDA:SGD.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IPI:SGD.
 GO:0000747; P:conjugation with cellular fusion; TAS:SGD.
 GO:0001403; P:invasive growth in response to glucose limitation; IMP:SGD.
 GO:0046020; P:negative regulation of transcription from RNA polymerase II promoter by pheromones; IMP:SGD.
 GO:0007329; P:positive regulation of transcription from RNA polymerase II promoter by pheromones; IMP:SGD.
 GO:0007124; P:pseudohyphal growth; IMP:SGD.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR003120; TF_STE. 
Pfam
 PF02200; STE 
SMART
 SM00424; STE 
PROSITE
  
PRINTS