CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004622
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Superkiller protein 3 
Protein Synonyms/Alias
  
Gene Name
 SKI3 
Gene Synonyms/Alias
 YPR189W; P9677.7 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
197SQNRLKLKASDPDYQubiquitination[1]
675ITETYASKPNWQAASubiquitination[1]
976DAAIFAFKKSIQLQSubiquitination[2]
977AAIFAFKKSIQLQSNubiquitination[2]
1088ILSNGRSKAAQFMYAmethylation[3]
1357LYRNDTGKQVWQRSAubiquitination[1]
1392IASNGQNKVTAEEMSubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269]
 [3] Identification of arginine- and lysine-methylation in the proteome of Saccharomyces cerevisiae and its functional implications.
 Pang CN, Gasteiger E, Wilkins MR.
 BMC Genomics. 2010 Feb 5;11:92. [PMID: 20137074] 
Functional Description
 Component of the SKI complex involved in 3'-mRNA degradation pathway. Represses dsRNA virus propagation by specifically blocking translation of viral mRNAs, perhaps recognizing the absence of CAP or poly(A). Essential for cell growth only in the presence of M1 replicon. 
Sequence Annotation
 REPEAT 4 37 TPR 1.
 REPEAT 47 80 TPR 2.
 REPEAT 425 458 TPR 3.
 REPEAT 471 507 TPR 4.
 REPEAT 508 541 TPR 5.
 REPEAT 627 661 TPR 6.
 REPEAT 702 735 TPR 7.
 REPEAT 736 769 TPR 8.
 REPEAT 945 985 TPR 9.
 REPEAT 987 1018 TPR 10.
 REPEAT 1226 1259 TPR 11.  
Keyword
 Antiviral defense; Complete proteome; Cytoplasm; Nucleus; Reference proteome; Repeat; TPR repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1432 AA 
Protein Sequence
MSDIKQLLKE AKQELTNRDY EETIEISEKV LKLDPDNYFA HIFLGKALSS LPASNNVSSN 60
RNLERATNHY VSAAKLVPDN LLAWKGLFLL FRTTEVVPDI LSYDEYFDLC GQYADALLKQ 120
EQSQVELIND IKLLKKTHPD CQKAFYQHLK PGSLMAETIG RHLSTPQDAL LNLIKILSNI 180
ETTEIGKTLS QNRLKLKASD PDYQIKLNSF SWEIIKNSEI DQLYNQLVNI LADDQKRSEI 240
ENQWLEYRIK VLKSMPLDVK KDFFTKVKEM VEDMVLVNHQ SLLAWQKYFE WTDYEDLDNM 300
DAPLIIKYFK KFPKDPLAMI LYSWLSSKLS KYDIKSLESA NKPPEGHKKT EKETDIKDVD 360
ETNEDEVKDR VEDEVKDRVE DEVKDQDEEA KEDEEEDLDD IEIGLLEEEV VTVLTENIVK 420
CKNNILAHRI LCQYYLLTKE YEAALPYIKN GISLIAYNIK DLGVHLPLTK REFSLDLATV 480
YTYVDAPKDH NAALKLYDNI LSGDFSNIQA KMGKGIIFIE RKNWKDAMTL LTQVHEQSPN 540
NLEVLSELSW SKAHMGYMDE ALAGLDTVIK GIKGMDLRSI DFRALNLWRQ AKVYIMKHAS 600
INDAKQENVK CAFKLLIQSI KILDTFAPGF STLGDIYCHY YKDHLRAFKC YFKAFDLDAG 660
DYTAAKYITE TYASKPNWQA ASSIASRLIK GEKAKAELRS NNWPFRVVGI AHLEKQEESD 720
SIEWFQSALR VDPNDVESWV GLGQAYHACG RIEASIKVFD KAIQLRPSHT FAQYFKAISL 780
CDVGEYLESL DILEKVCQEA ATEESFQIGL VEVLMRCSLD LYSQGFLLKS VSIAKDTIER 840
IKIIISELKC ENQQVWIYLS QVLRLFIWIE SKVDTLPVES LVSIFENSQF SGSEEIDSVD 900
NIKIDTLLDS TTDDNVSIAC KFLILASKYS VSDQKFTDIA GTVRASYWYN IGISELTAFI 960
TLKEPQYRDA AIFAFKKSIQ LQSNTSETWI GLGIATMDIN FRVSQHCFIK ATALEPKATN 1020
TWFNLAMLGL KKKDTEFAQQ VLNKLQSLAP QDSSPWLGMA LILEEQGDII GSSKLFAHSF 1080
ILSNGRSKAA QFMYAKNVLE NHINNGDDER DIETVEKLTT ASIALEQFFK KSPDSQFALQ 1140
CALLTLERLH HYENANELAN RLIGILEKKF EKTQDERELF NFAIIKGQFA RIHLGLGNFE 1200
LSIENADLSQ GIISESSDEK SMKTKISNHI CLGLSYFFLN DFDQTLNQFQ ELLSISKDSK 1260
HLVVLIAKVL YDVGESDTKE IALQELTEYI ATSGADLLVT LTIAAMSILD DKREDLSIIL 1320
EELKALPLSK QIIDKHKDAP YLIEEITKRL YRNDTGKQVW QRSAYFFPNN LKVWERLDKN 1380
IQRRIASNGQ NKVTAEEMSK LYCESKNLRS IQRGMFLCPW NVTAVKALNE CF 1432 
Gene Ontology
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0055087; C:Ski complex; IDA:SGD.
 GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
 GO:0070478; P:nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay; IMP:SGD.
 GO:0070481; P:nuclear-transcribed mRNA catabolic process, non-stop decay; IMP:SGD. 
Interpro
 IPR001440; TPR-1.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR019734; TPR_repeat. 
Pfam
 PF00515; TPR_1 
SMART
 SM00028; TPR 
PROSITE
 PS50005; TPR
 PS50293; TPR_REGION 
PRINTS