UniProt Accession

 EIF3I_MOUSE; Q9QZD9 

Genbank Protein ID

 AF188297; AF271072; AK012375; AK002896; AK010781; BC029625 

Genbank Nucleotide ID

 AAF01455.1; AAF76199.1; BAB28197.1; BAB22440.1; BAB27177.1; AAH29625.1 

Protein Name

 Eukaryotic translation initiation factor 3 subunit I 

Protein Synonyms/Alias

 eIF3i; Eukaryotic translation initiation factor 3 subunit 2; TGF-beta receptor-interacting protein 1; TRIP-1; eIF-3-beta; eIF3 p36 

Gene Name

 Eif3i 

Gene Synonyms/Alias

 Eif3s2; Trip1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
2	******MKPILLQGH	acetylation	[1]
2	******MKPILLQGH	ubiquitination	[2]
224	STTLEHQKTFRTERP	ubiquitination	[2]
282	EEEFGRVKGHFGPIN	ubiquitination	[2]
298	VAFHPDGKSYSSGGE	ubiquitination	[2]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. 

Sequence Annotation

 REPEAT 8 47 WD 1.
 REPEAT 50 91 WD 2.
 REPEAT 144 183 WD 3.
 REPEAT 186 225 WD 4.
 REPEAT 283 324 WD 5.
 MOD_RES 264 264 N6-acetyllysine (By similarity).
 MOD_RES 308 308 Phosphotyrosine (By similarity).
 CROSSLNK 282 282 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Initiation factor; Isopeptide bond; Phosphoprotein; Protein biosynthesis; Reference proteome; Repeat; Ubl conjugation; WD repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 325 AA 

Protein Sequence

Gene Ontology

 GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:HAMAP.
 GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:HAMAP.
 GO:0005852; C:eukaryotic translation initiation factor 3 complex; IDA:UniProtKB.
 GO:0003743; F:translation initiation factor activity; IEA:HAMAP.
 GO:0001731; P:formation of translation preinitiation complex; IEA:HAMAP.
 GO:0006446; P:regulation of translational initiation; IEA:HAMAP.
 GO:0006413; P:translational initiation; IDA:UniProtKB. 

Interpro

 IPR027525; eIF3i.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 

Pfam

 PF00400; WD40 

SMART

 SM00320; WD40 

PROSITE

 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 

PRINTS