CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-033435
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 AP-2 complex subunit mu 
Protein Synonyms/Alias
  
Gene Name
 AP2M1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
139FITQQGIKSQHQTKEubiquitination[1]
164GTADETSKSGKQSIAubiquitination[1]
167DETSKSGKQSIAIDDubiquitination[2, 3, 4]
185HQCVRLSKFDSERSIacetylation[4, 5]
185HQCVRLSKFDSERSIubiquitination[1, 3, 4, 6]
210LMRYRTTKDIILPFRubiquitination[3, 4, 6]
241VVIKSNFKPSLLAQKubiquitination[1, 3, 4, 6]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 252 AA 
Protein Sequence
MIGGLFIYNH KGEVLISRVY RDDIGRNAVD AFRVNVIHAR QQVRSPVTNI ARTSFFHVKR 60
SNIWLAAVTK QNVNAAMVFE FLYKMCDVMA AYFGKISEEN IKNNFVLIYE LLDEILDFGY 120
PQNSETGALK TFITQQGIKS QHQTKEEQSQ ITSQGKGTAD ETSKSGKQSI AIDDCTFHQC 180
VRLSKFDSER SISFIPPDGE FELMRYRTTK DIILPFRVIP LVREVGRTKL EVKVVIKSNF 240
KPSLLAQKIE VR 252 
Gene Ontology
 GO:0030131; C:clathrin adaptor complex; IEA:InterPro.
 GO:0030132; C:clathrin coat of coated pit; IEA:InterPro.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0016192; P:vesicle-mediated transport; IEA:InterPro. 
Interpro
 IPR022775; AP_mu_sigma_su.
 IPR015629; Clathrin_AP50.
 IPR001392; Clathrin_mu.
 IPR008968; Clathrin_mu_C.
 IPR018240; Clathrin_mu_CS.
 IPR011012; Longin-like_dom. 
Pfam
 PF00928; Adap_comp_sub
 PF01217; Clat_adaptor_s 
SMART
  
PROSITE
 PS00991; CLAT_ADAPTOR_M_2
 PS51072; MHD 
PRINTS
 PR00314; CLATHRINADPT.