CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-027293
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic translation initiation factor 3 subunit B 
Protein Synonyms/Alias
 eIF3b; Eukaryotic translation initiation factor 3 subunit 9; eIF-3-eta 
Gene Name
 Eif3b 
Gene Synonyms/Alias
 Eif3s9 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
98QEPSAPGKAEAVGEQubiquitination[1]
191VGPDRLEKLKNVIHKubiquitination[1]
198KLKNVIHKIFSKFGKubiquitination[1]
202VIHKIFSKFGKIINDubiquitination[1]
205KIFSKFGKIINDYYPubiquitination[1]
237AHAVDAVKNADGYKLubiquitination[1]
246ADGYKLDKQHTFRVNubiquitination[1]
273DEWDIPEKQPFKDLGubiquitination[1]
277IPEKQPFKDLGNLRYubiquitination[1]
313SIFWNDVKDPVSIEEubiquitination[1]
353IALWGGDKFKQIQRFubiquitination[1]
448PSMGLLDKKSLKISGubiquitination[1]
505LFNVVDCKLHWQKNGubiquitination[1]
524VKVDRTPKGTQGVVTubiquitination[1]
541EIFRMREKQVPVDVVubiquitination[1]
718KDLKKYSKIFEQKDRubiquitination[1]
759MAQELYMKQKNERLEubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (By similarity). 
Sequence Annotation
 DOMAIN 174 257 RRM (By similarity).
 REPEAT 321 359 WD 1 (By similarity).
 REPEAT 361 406 WD 2 (By similarity).
 REPEAT 410 448 WD 3 (By similarity).
 REPEAT 549 590 WD 4 (By similarity).
 REPEAT 638 683 WD 5 (By similarity).
 REGION 113 402 Sufficient for interaction with EIF3E (By
 REGION 159 263 Sufficient for interaction with EIF3J (By
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 79 79 Phosphoserine (By similarity).
 MOD_RES 141 141 Phosphoserine (By similarity).
 MOD_RES 143 143 Phosphoserine (By similarity).
 MOD_RES 153 153 Phosphoserine (By similarity).  
Keyword
 Acetylation; Cytoplasm; Initiation factor; Phosphoprotein; Protein biosynthesis; Repeat; RNA-binding; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 970 AA 
Protein Sequence
MQDAENVAVP EAAEERAEPA RQQPASESPP TDEAAGSGGS EVGQTEDAEE DAEAGPEPEV 60
RAKPAAQSEE ETATSPAASP TPQSAERSPS QEPSAPGKAE AVGEQARGHP SAGAEEEGGS 120
DGSAAEAEPR ALENGEADEP SFSDPEDFVD DVSEEELLGD VLKDRPQEAD GIDSVIVVDN 180
VPQVGPDRLE KLKNVIHKIF SKFGKIINDY YPEEDGKTKG YIFLEYASPA HAVDAVKNAD 240
GYKLDKQHTF RVNLFTDFDK YMTISDEWDI PEKQPFKDLG NLRYWLEEAE CRDQYSVIFE 300
SGDRTSIFWN DVKDPVSIEE RARWTETYVR WSPKGTYLAT FHQRGIALWG GDKFKQIQRF 360
SHQGVQLIDF SPCERYLVTF SPLMDTQDDP QAIIIWDILT GHKKRGFHCE SSAHWPIFKW 420
SHDGKFFARM TLDTLSIYET PSMGLLDKKS LKISGIKDFS WSPGGNIIAF WVPEDKDIPA 480
RVTLMQLPTR QEIRVRNLFN VVDCKLHWQK NGDYLCVKVD RTPKGTQGVV TNFEIFRMRE 540
KQVPVDVVEM KETIIAFAWE PNGSKFAVLH GEAPRISVSF YHVKSNGKIE LIKMFDKQQA 600
NTIFWSPQGQ FVVLAGLRSM NGALAFVDTS DCTVMNIAEH YMASDVEWDP TGRYVVTSVS 660
WWSHKVDNAY WLWTFQGRLL QKNNKDRFCQ LLWRPRPPTL LSQDQIKQIK KDLKKYSKIF 720
EQKDRLSQSK ASKELVERRR TMMEDFRQYR KMAQELYMKQ KNERLELRGG VDTDELDSNV 780
DDWEEETIEF FCHRGSHSSG KSGVISALCV TVPCPGSEAT LHGEPRAGLL PVLSCSGLRV 840
HPGFFYPDTA LCLQPQVHSG DTRASALTLP CLGFYQPCLH WSLTAVFLVA PLNSCCCPWG 900
FQSLHLQLSG VSIHGLAVPV GQCRVAWRPS GVCTPTTYLC QKLWRHPHLP PVQVVAASVQ 960
VGCGNKSNPV 970 
Gene Ontology
 GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:HAMAP.
 GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:HAMAP.
 GO:0005852; C:eukaryotic translation initiation factor 3 complex; IEA:HAMAP.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003743; F:translation initiation factor activity; IEA:HAMAP.
 GO:0001731; P:formation of translation preinitiation complex; IEA:HAMAP.
 GO:0006446; P:regulation of translational initiation; IEA:HAMAP. 
Interpro
 IPR011042; 6-blade_b-propeller_TolB-like.
 IPR011400; EIF3B.
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom.
 IPR013979; TIF_beta_prop-like.
 IPR015943; WD40/YVTN_repeat-like_dom. 
Pfam
 PF08662; eIF2A
 PF00076; RRM_1 
SMART
 SM00360; RRM 
PROSITE
 PS50102; RRM 
PRINTS