CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000247
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Citron Rho-interacting kinase 
Protein Synonyms/Alias
 CRIK; Serine/threonine-protein kinase 21 
Gene Name
 CIT 
Gene Synonyms/Alias
 CRIK; KIAA0949; STK21 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
71QPALMKIKHVSNFVRubiquitination[1]
126TGDIYAMKVMKKKALubiquitination[1]
236VDRTGHIKLVDFGSAubiquitination[1]
245VDFGSAAKMNSNKMVubiquitination[1]
323MNFQRFLKFPDDPKVubiquitination[1]
350CGQKERLKFEGLCCHubiquitination[1]
1613GGRVSREKAEADAKLubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Plays a role in cytokinesis. Required for KIF14 localization to the central spindle and midbody. Putative RHO/RAC effector that binds to the GTP-bound forms of RHO and RAC1. It probably binds p21 with a tighter specificity in vivo. Dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues. Plays an important role in the regulation of cytokinesis and the development of the central nervous system. Phosphorylates MYL9/MLC2. 
Sequence Annotation
 DOMAIN 97 360 Protein kinase.
 DOMAIN 361 431 AGC-kinase C-terminal.
 DOMAIN 1443 1563 PH.
 DOMAIN 1591 1881 CNH.
 NP_BIND 103 111 ATP (By similarity).
 ZN_FING 1362 1411 Phorbol-ester/DAG-type.
 REGION 1091 1302 Interaction with Rho/Rac.
 MOTIF 1953 1958 SH3-binding (Potential).
 ACT_SITE 221 221 Proton acceptor (By similarity).
 BINDING 126 126 ATP (By similarity).
 MOD_RES 433 433 Phosphoserine.
 MOD_RES 440 440 Phosphoserine.
 MOD_RES 480 480 Phosphoserine (By similarity).
 MOD_RES 1196 1196 Phosphotyrosine (By similarity).
 MOD_RES 1478 1478 Phosphotyrosine (By similarity).
 MOD_RES 1582 1582 Phosphoserine (By similarity).
 MOD_RES 1721 1721 N6-acetyllysine.  
Keyword
 Acetylation; Alternative splicing; ATP-binding; Cell cycle; Cell division; Coiled coil; Complete proteome; Cytoplasm; Developmental protein; Differentiation; Kinase; Metal-binding; Mitosis; Neurogenesis; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; SH3-binding; Transferase; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2027 AA 
Protein Sequence
MLKFKYGARN PLDAGAAEPI ASRASRLNLF FQGKPPFMTQ QQMSPLSREG ILDALFVLFE 60
ECSQPALMKI KHVSNFVRKY SDTIAELQEL QPSAKDFEVR SLVGCGHFAE VQVVREKATG 120
DIYAMKVMKK KALLAQEQVS FFEEERNILS RSTSPWIPQL QYAFQDKNHL YLVMEYQPGG 180
DLLSLLNRYE DQLDENLIQF YLAELILAVH SVHLMGYVHR DIKPENILVD RTGHIKLVDF 240
GSAAKMNSNK MVNAKLPIGT PDYMAPEVLT VMNGDGKGTY GLDCDWWSVG VIAYEMIYGR 300
SPFAEGTSAR TFNNIMNFQR FLKFPDDPKV SSDFLDLIQS LLCGQKERLK FEGLCCHPFF 360
SKIDWNNIRN SPPPFVPTLK SDDDTSNFDE PEKNSWVSSS PCQLSPSGFS GEELPFVGFS 420
YSKALGILGR SESVVSGLDS PAKTSSMEKK LLIKSKELQD SQDKCHKMEQ EMTRLHRRVS 480
EVEAVLSQKE VELKASETQR SLLEQDLATY ITECSSLKRS LEQARMEVSQ EDDKALQLLH 540
DIREQSRKLQ EIKEQEYQAQ VEEMRLMMNQ LEEDLVSARR RSDLYESELR ESRLAAEEFK 600
RKATECQHKL LKAKDQGKPE VGEYAKLEKI NAEQQLKIQE LQEKLEKAVK ASTEATELLQ 660
NIRQAKERAE RELEKLQNRE DSSEGIRKKL VEAEERRHSL ENKVKRLETM ERRENRLKDD 720
IQTKSQQIQQ MADKILELEE KHREAQVSAQ HLEVHLKQKE QHYEEKIKVL DNQIKKDLAD 780
KETLENMMQR HEEEAHEKGK ILSEQKAMIN AMDSKIRSLE QRIVELSEAN KLAANSSLFT 840
QRNMKAQEEM ISELRQQKFY LETQAGKLEA QNRKLEEQLE KISHQDHSDK NRLLELETRL 900
REVSLEHEEQ KLELKRQLTE LQLSLQERES QLTALQAARA ALESQLRQAK TELEETTAEA 960
EEEIQALTAH RDEIQRKFDA LRNSCTVITD LEEQLNQLTE DNAELNNQNF YLSKQLDEAS 1020
GANDEIVQLR SEVDHLRREI TEREMQLTSQ KQTMEALKTT CTMLEEQVMD LEALNDELLE 1080
KERQWEAWRS VLGDEKSQFE CRVRELQRML DTEKQSRARA DQRITESRQV VELAVKEHKA 1140
EILALQQALK EQKLKAESLS DKLNDLEKKH AMLEMNARSL QQKLETEREL KQRLLEEQAK 1200
LQQQMDLQKN HIFRLTQGLQ EALDRADLLK TERSDLEYQL ENIQVLYSHE KVKMEGTISQ 1260
QTKLIDFLQA KMDQPAKKKK GLFSRRKEDP ALPTQVPLQY NELKLALEKE KARCAELEEA 1320
LQKTRIELRS AREEAAHRKA TDHPHPSTPA TARQQIAMSA IVRSPEHQPS AMSLLAPPSS 1380
RRKESSTPEE FSRRLKERMH HNIPHRFNVG LNMRATKCAV CLDTVHFGRQ ASKCLECQVM 1440
CHPKCSTCLP ATCGLPAEYA THFTEAFCRD KMNSPGLQTK EPSSSLHLEG WMKVPRNNKR 1500
GQQGWDRKYI VLEGSKVLIY DNEAREAGQR PVEEFELCLP DGDVSIHGAV GASELANTAK 1560
ADVPYILKME SHPHTTCWPG RTLYLLAPSF PDKQRWVTAL ESVVAGGRVS REKAEADAKL 1620
LGNSLLKLEG DDRLDMNCTL PFSDQVVLVG TEEGLYALNV LKNSLTHVPG IGAVFQIYII 1680
KDLEKLLMIA GEERALCLVD VKKVKQSLAQ SHLPAQPDIS PNIFEAVKGC HLFGAGKIEN 1740
GLCICAAMPS KVVILRYNEN LSKYCIRKEI ETSEPCSCIH FTNYSILIGT NKFYEIDMKQ 1800
YTLEEFLDKN DHSLAPAVFA ASSNSFPVSI VQVNSAGQRE EYLLCFHEFG VFVDSYGRRS 1860
RTDDLKWSRL PLAFAYREPY LFVTHFNSLE VIEIQARSSA GTPARAYLDI PNPRYLGPAI 1920
SSGAIYLASS YQDKLRVICC KGNLVKESGT EHHRGPSTSR SSPNKRGPPT YNEHITKRVA 1980
SSPAPPEGPS HPREPSTPHR YREGRTELRR DKSPGRPLER EKSPGRMLST RRERSPGRLF 2040
EDSSRGRLPA GAVRTPLSQV NKVWDQSSV 2069 
Gene Ontology
 GO:0015629; C:actin cytoskeleton; IEA:Compara.
 GO:0001726; C:ruffle; IEA:Compara.
 GO:0005773; C:vacuole; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0004674; F:protein serine/threonine kinase activity; ISS:UniProtKB.
 GO:0005083; F:small GTPase regulator activity; IEA:InterPro.
 GO:0000910; P:cytokinesis; IMP:UniProtKB.
 GO:0016358; P:dendrite development; IEA:Compara.
 GO:0048699; P:generation of neurons; ISS:UniProtKB.
 GO:0035556; P:intracellular signal transduction; IEA:InterPro.
 GO:0007091; P:metaphase/anaphase transition of mitotic cell cycle; IEA:Compara.
 GO:0007067; P:mitosis; ISS:UniProtKB.
 GO:0000070; P:mitotic sister chromatid segregation; IEA:Compara.
 GO:0050774; P:negative regulation of dendrite morphogenesis; IEA:Compara.
 GO:0007283; P:spermatogenesis; IEA:Compara. 
Interpro
 IPR000961; AGC-kinase_C.
 IPR001180; Citron.
 IPR017405; Citron_Rho-interacting_kinase.
 IPR011009; Kinase-like_dom.
 IPR011993; PH_like_dom.
 IPR017892; Pkinase_C.
 IPR001849; Pleckstrin_homology.
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00780; CNH
 PF00069; Pkinase
 PF00433; Pkinase_C 
SMART
 SM00109; C1
 SM00036; CNH
 SM00233; PH
 SM00133; S_TK_X
 SM00220; S_TKc 
PROSITE
 PS51285; AGC_KINASE_CTER
 PS50219; CNH
 PS50003; PH_DOMAIN
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 
PRINTS