CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011204
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin ligase complex F-box protein UFO1 
Protein Synonyms/Alias
  
Gene Name
 UFO1 
Gene Synonyms/Alias
 YML088W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
180FGKLLSNKSYLTPVMubiquitination[1]
465HHHSTKGKNTVSNKWacetylation[2]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Preparative peptide isoelectric focusing as a tool for improving the identification of lysine-acetylated peptides from complex mixtures.
 Xie H, Bandhakavi S, Roe MR, Griffin TJ.
 J Proteome Res. 2007 May;6(5):2019-26. [PMID: 17397211] 
Functional Description
 Substrate recognition component of a SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins (By similarity). 
Sequence Annotation
 DOMAIN 5 51 F-box.
 REPEAT 547 566 UIM 1.
 REPEAT 583 602 UIM 2.
 REPEAT 651 668 UIM 3.
 MOD_RES 511 511 Phosphoserine.
 MOD_RES 514 514 Phosphothreonine.  
Keyword
 Complete proteome; Phosphoprotein; Reference proteome; Repeat; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 668 AA 
Protein Sequence
MERPGLVLQD LPPEILINIF SHLDEKDLFT LQELSTHFRN LIHDEELWKN LFKSRVHTTH 60
FPTFSQSSKF SVEYIERTRG LHHWQHNKAI RTKYTIIPTR NWDQPSIERI VFDYPRVAAY 120
NDGTITILQL QNHKRQKKFK KLIYIPCTTP QGCSTMDFNI NAAVFGRFDG RVFGKLLSNK 180
SYLTPVMEFT GRHSAGVTAI CNSESWDTSR EDWSVSGSEN GEIIWWCENK LVKMWKVSNR 240
VIWKLAFFKD WTLIMDDEKL YIIHQMQELH SIDIPKDLDE QPMRVRFFKM DFGSMTLVLA 300
DLNNVYTISV NPNGNFGNLR KLEMPEQICA VEIDEKTSQR EQNWQFAGDD GCYISLLTTQ 360
NTLYIINIRD LSSSGLKVQC KISFDEQVYV SQVTNLIVVV ALPNVLQILN AMTGELIKTV 420
LKTEKFPEFL KVSQDKIIMG SGNVLNYLKF VSSDSKKHHH STKGKNTVSN KWNETLNTEL 480
QYYDEDEDLR RKRQSEISRL IDAYGGDLEL SGDTDEENDI QLRIALLESQ EAQARNQAEA 540
GEPVGDDEDE QLRRALEESQ LIYETQTNSS ANHGNNTNDE IDEDDEEFLR AIRQSRVEDE 600
RRRHLRNHTT GRRNGPLSDD NFATYGAAES SERTSTENTI GSSVGVDASN NVDEDLQLAI 660
ALSLSEIN 668 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0019005; C:SCF ubiquitin ligase complex; IDA:SGD.
 GO:0030674; F:protein binding, bridging; ISA:SGD.
 GO:0071406; P:cellular response to methylmercury; IMP:SGD.
 GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
 GO:0006974; P:response to DNA damage stimulus; IMP:SGD.
 GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IMP:SGD. 
Interpro
 IPR001810; F-box_dom_cyclin-like.
 IPR003903; Ubiquitin-int_motif.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00646; F-box 
SMART
 SM00256; FBOX
 SM00726; UIM 
PROSITE
 PS50181; FBOX
 PS50330; UIM 
PRINTS