CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018763
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ATPase family AAA domain-containing protein 3 
Protein Synonyms/Alias
 AAA-ATPase TOB3 
Gene Name
 Atad3 
Gene Synonyms/Alias
 Atad3a; Kiaa1273 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
134ARAQYQDKLARQRYEacetylation[1]
362TGKTLFAKKLALHSGacetylation[1]
393EGVTAMHKVFDWASTacetylation[1]
423LRKRATEKISEDLRAacetylation[2, 3]
423LRKRATEKISEDLRAsuccinylation[3]
490LVRMYFDKYVLKPATacetylation[1, 2, 3, 4, 5, 6, 7, 8, 9]
490LVRMYFDKYVLKPATsuccinylation[3]
494YFDKYVLKPATEGKQacetylation[1, 2, 9]
512VAQFDYGKKCSEVAQacetylation[2, 4]
567AVQQHQQKMQWLKVEacetylation[1]
Reference
 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
 [5] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [6] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]
 [7] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [8] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [9] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654
Functional Description
 Essential for mitochondrial network organization, mitochondrial metabolism and cell growth at organism and cellular level. May play an important in mitochondrial protein synthesis. May also participate in mitochondrial DNA replication. May bind to mitochondrial DNA D-loops and contribute to nucleoid stability. Required for enhanced channeling of cholesterol for hormone- dependent steroidogenesis (By similarity). 
Sequence Annotation
 NP_BIND 351 358 ATP (Potential).
 REGION 1 49 Required for interaction with the inner
 REGION 289 304 S100B-binding (By similarity).
 MOD_RES 490 490 N6-acetyllysine.
 MOD_RES 512 512 N6-acetyllysine.  
Keyword
 Acetylation; Alternative splicing; ATP-binding; Coiled coil; Complete proteome; Direct protein sequencing; Membrane; Mitochondrion; Mitochondrion inner membrane; Mitochondrion nucleoid; Nucleotide-binding; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 591 AA 
Protein Sequence
MSWLFGIKGP KGEGTGPPLP LPPAQPGAEG GGDRGAGDRP SPKDKWSNFD PTGLERAAKA 60
ARELEHSRHA KEALSLAQMQ EQTLQLEQQS KLKEYEAAVE QLKSEQIRVQ AEERRKTLTE 120
ETRQHQARAQ YQDKLARQRY EDQLKQQQLL NEENLRKQEE SVQKQEAIRR ATVEREMELR 180
HKNEMLRVEA EARARAKADR ENADIIREQI RLKAAEHRQT ILESIRTAGT LLGEGFRAFV 240
TDWDKVTATV AGLTLLAVGV YSAKNATSVA GRYIEARLGK PSLVRETSRI SVLEALRHPI 300
QVSRRLVSRP QDALEGVILS PSLEARVRDI AIATRNTKKN KSLYRNVLMY GPPGTGKTLF 360
AKKLALHSGM DYAIMTGGDV APMGREGVTA MHKVFDWAST SRRGLLLFVD EADAFLRKRA 420
TEKISEDLRA TLNAFLHRTG QHSSKFMLVL ASNQPEQFDW AINDRIDEMV CFALPQREER 480
ERLVRMYFDK YVLKPATEGK QRLKVAQFDY GKKCSEVAQL TEGMSGREIA QLAVAWQAMA 540
YSSEDGVLTE AMMDARVQDA VQQHQQKMQW LKVERPDSQT NKPPHPSLLS C 591 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0042645; C:mitochondrial nucleoid; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0017111; F:nucleoside-triphosphatase activity; IEA:InterPro. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR003959; ATPase_AAA_core.
 IPR021911; DUF3523.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00004; AAA
 PF12037; DUF3523 
SMART
 SM00382; AAA 
PROSITE
  
PRINTS