UniProt Accession

 EFHD2_HUMAN; Q96C19; Q5JYW9 

Genbank Protein ID

 AL031283; CH471167; BC007233; BC014923; BC023611; BC068473 

Genbank Nucleotide ID

 CAI21431.1; EAW51720.1; AAH07233.1; AAH14923.1; AAH23611.1; AAH68473.1 

Protein Name

 EF-hand domain-containing protein D2 

Protein Synonyms/Alias

 Swiprosin-1 

Gene Name

 EFHD2 

Gene Synonyms/Alias

 SWS1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
9	ATDELATKLSRRLQM	acetylation	[1, 2]
9	ATDELATKLSRRLQM	ubiquitination	[3]
87	FNPYTEFKEFSRKQI	ubiquitination	[3, 4, 5]
102	KDMEKMFKQYDAGRD	ubiquitination	[3]
138	LGLKNMIKEVDEDFD	ubiquitination	[3]
188	DVSSEGVKGAKSFFE	ubiquitination	[3]
191	SEGVKGAKSFFEAKV	ubiquitination	[3, 6]
233	KQRKAAFKELQSTFK	acetylation	[2, 7, 8]
233	KQRKAAFKELQSTFK	ubiquitination	[3]

Reference

 [1] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [7] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [8] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 

Functional Description

 May regulate B-cell receptor (BCR)-induced immature and primary B-cell apoptosis (By similarity). Plays a role as negative regulator of the canonical NF-kappa-B-activating branch (By similarity). Controls spontaneous apoptosis through the regulation of BCL2L1 abundance (By similarity). 

Sequence Annotation

 DOMAIN 92 127 EF-hand 1.
 DOMAIN 128 163 EF-hand 2.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 11 11 Phosphoserine.
 MOD_RES 74 74 Phosphoserine.
 MOD_RES 76 76 Phosphoserine.
 MOD_RES 83 83 Phosphotyrosine (By similarity).
 MOD_RES 233 233 N6-acetyllysine.  

Keyword

 Acetylation; Calcium; Complete proteome; Direct protein sequencing; Membrane; Metal-binding; Phosphoprotein; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 240 AA 

Protein Sequence

Gene Ontology

 GO:0045121; C:membrane raft; IEA:UniProtKB-SubCell.
 GO:0005509; F:calcium ion binding; IEA:Compara. 

Interpro

 IPR011992; EF-hand-like_dom.
 IPR002048; EF_hand_dom. 

Pfam

 PF13499; EF_hand_5 

SMART

 SM00054; EFh 

PROSITE

 PS00018; EF_HAND_1
 PS50222; EF_HAND_2 

PRINTS