CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-036902
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Unconventional myosin-Ib 
Protein Synonyms/Alias
  
Gene Name
 MYO1B 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
125TLQRIVQKYFLEMKNubiquitination[1]
197SELFKDKKALYPSSVubiquitination[2]
217GAYLEINKNPKYKKLubiquitination[1, 2, 3, 4, 5]
231LKDAIEEKIIIAEVVubiquitination[1, 2, 3, 4, 5, 6]
240IIAEVVNKINRANGKubiquitination[1]
266NLLLADQKSGQIKSEubiquitination[1]
331RTTLSQTKQKLNIEIubiquitination[3, 4]
361KFIQGNQKNGSVPTCubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 381 AA 
Protein Sequence
MKKSQIVIAA WYRRYAQQKR YQQTKSSALV IQSYIRGWKA RKILRELKHQ KRCKEAVTTI 60
AAYWHGTQAR RELKRLKEEA RRKHAVAVIW AYWLGLKVRR EYRKFFRANA GKKIYEFTLQ 120
RIVQKYFLEM KNKMPSLSPI DKNWPSRPYL FLDSTHKELK RIFHLWRCKK YRDQFTDQQK 180
LIYEEKLEAS ELFKDKKALY PSSVGQPFQG AYLEINKNPK YKKLKDAIEE KIIIAEVVNK 240
INRANGKSTS RIFLLTNNNL LLADQKSGQI KSEVPLVDVT KVSMSSQNDG FFAVHLKEGS 300
EAASKGDFLF SSDHLIEMAT KLYRTTLSQT KQKLNIEISD EFLVQFRQDK VCVKFIQGNQ 360
KNGSVPTCKR KNNRLLEVAV P 381 
Gene Ontology
 GO:0016459; C:myosin complex; IEA:InterPro.
 GO:0003774; F:motor activity; IEA:InterPro. 
Interpro
 IPR000048; IQ_motif_EF-hand-BS.
 IPR010926; Myosin_tail_2.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00612; IQ
 PF06017; Myosin_TH1 
SMART
 SM00015; IQ 
PROSITE
 PS50096; IQ 
PRINTS