CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017377
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Poly [ADP-ribose] polymerase 8 
Protein Synonyms/Alias
 PARP-8; ADP-ribosyltransferase diphtheria toxin-like 16; ARTD16 
Gene Name
 PARP8 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
20DIDVVIQKSRAEKDCubiquitination[1, 2]
372RPCPAAVKSEECLTLacetylation[3]
380SEECLTLKSHRLLTRacetylation[3]
405NTNLKPHKLLSRSYSacetylation[3]
427LYGLKNHKLLSKSYSubiquitination[4]
431KNHKLLSKSYSSAPKubiquitination[4]
441SSAPKSSKTELFKEPubiquitination[4]
607KNYDRVMKALDSITSubiquitination[4]
654SNRSHIVKLPVNRQLubiquitination[4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
  
Sequence Annotation
 DOMAIN 617 844 PARP catalytic.  
Keyword
 Alternative splicing; Complete proteome; Glycosyltransferase; NAD; Polymorphism; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 854 AA 
Protein Sequence
MGMCSRQERI QKDIDVVIQK SRAEKDCLFA DFRYSDSTFT FTYVGGPRSV SYSVHVSEDY 60
PDNTYVSSSE NDEDVLVTTE PIPVIFHRIA TELRKTNDIN CCLSIKSKLQ KENGEESRQN 120
STVEEDSEGD NDSEEFYYGG QVNYDGELHK HPQLEADLSA VREIYGPHAV SLREYGAIDD 180
VDIDLHIDVS FLDEEIAVAW EVIRTEPIIV RLHCSLTQYL NGPVPTVDVF QISTKERFGL 240
GHQLKKIMQT FVTQQWKQSK EKSNCLHNKK LSEKKVKSPL HLFSTLRRSP SYPPPGCGKS 300
KSKLKSEQDG ISKTHKLLRR TCSSTVKTDD VCVTKSHRTF GRSLSSDPRA EQAMTAIKSH 360
KLLNRPCPAA VKSEECLTLK SHRLLTRSCS GDPRCEHNTN LKPHKLLSRS YSSNLRMEEL 420
YGLKNHKLLS KSYSSAPKSS KTELFKEPNA EGRRLSLTSG LIGILTPSSS SSSQLAPNGA 480
KCIPVRDRGF LVQTIEFAEQ RIPVLNEYCV VCDEPHVFQN GPMLRPTVCE RELCVFAFQT 540
LGVMNEAADE IATGAQVVDL LVSMCRSALE SPRKVVIFEP YPSVVDPNDP QMLAFNPRKK 600
NYDRVMKALD SITSIREMTQ APYLEIKKQM DKQDPLAHPL LQWVISSNRS HIVKLPVNRQ 660
LKFMHTPHQF LLLSSPPAKE SNFRAAKKLF GSTFAFHGSH IENWHSILRN GLVVASNTRL 720
QLHGAMYGSG IYLSPMSSIS FGYSGMNKKQ KVSAKDEPAS SSKSSNTSQS QKKGQQSQFL 780
QSRNLKCIAL CEVITSSDLH KHGEIWVVPN TDHVCTRFFF VYEDGQVGDA NINTQEGGIH 840
KEILRVIGNQ TATG 854 
Gene Ontology
 GO:0005622; C:intracellular; IDA:LIFEdb.
 GO:0003950; F:NAD+ ADP-ribosyltransferase activity; IEA:EC. 
Interpro
 IPR012317; Poly(ADP-ribose)pol_cat_dom. 
Pfam
 PF00644; PARP 
SMART
  
PROSITE
 PS51059; PARP_CATALYTIC 
PRINTS