| Tag | Content |
|---|
| CPLM ID | CPLM-011362 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Sphingoid long chain base kinase 5 |
Protein Synonyms/Alias | LCB kinase 5; Sphinganine kinase 5 |
Gene Name | LCB5 |
Gene Synonyms/Alias | YLR260W |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 113 | TSTTDTSKDDLSANP | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] |
Functional Description | Catalyzes the phosphorylation of the sphingoid long chain bases dihydrosphingosine (DHS or sphinganine) and phytosphingosine (PHS) to form dihydrosphingosine 1-phosphate (DHS-1P) and phytosphingosine 1-phosphate (PHS-1P) respectively. Redundant to LCB4, is only responsible for few percent of the total activity. Involved in the biosynthesis of sphingolipids and ceramides. Involved in heat-induced transient cell cycle arrest. Accumulation of phosphorylated sphingoid long chain bases (LCBPs) stimulates calcium influx and activates calcineurin signaling. Involved in heat-stress resistance. |
Sequence Annotation | DOMAIN 266 405 DAGKc.
LIPID 91 91 S-palmitoyl cysteine (By similarity).
LIPID 94 94 S-palmitoyl cysteine (By similarity). |
Keyword | ATP-binding; Complete proteome; Golgi apparatus; Kinase; Lipid metabolism; Lipoprotein; Membrane; Nucleotide-binding; Palmitate; Reference proteome; Sphingolipid metabolism; Transferase. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 687 AA |
Protein Sequence | MTLKPSKRRK GRSRHSRKKQ ITSAILTEEG IMIKAKPSSP YTYANRMADK RSRSSIDNIS 60
RTSFQSNISR TSFQSNSDNN SIFETASLIS CVTCLSDTDT IDRSETSTTD TSKDDLSANP 120
KLHYPSVNGQ LPANTVIPYG RILDARYIEK EPLHYYDANS SPSSPLSSSM SNISEKCDLD 180
ELESSQKKER KGNSLSRGSN SSSSLLTSRS PFTKLVEVIF ARPRRHDVVP KRVSLYIDYK 240
PHSSSHLKEE DDLVEEILKR SYKNTRRNKS IFVIINPFGG KGKAKKLFMT KAKPLLLASR 300
CSIEVVYTKY PGHAIEIARE MDIDKYDTIA CASGDGIPHE VINGLYQRPD HVKAFNNIAI 360
TEIPCGSGNA MSVSCHWTNN PSYSTLCLIK SIETRIDLMC CSQPSYAREH PKLSFLSQTY 420
GLIAETDINT EFIRWMGPAR FELGVAFNII QKKKYPCEIY VKYAAKSKNE LKNHYLEHKN 480
KGSLEFQHIT MNKDNEDCDN YNYENEYETE NEDEDEDADA DDEDSHLISR DLADSSADQI 540
KEEDFKIKYP LDEGIPSDWE RLDPNISNNL GIFYTGKMPY VAADTKFFPA ALPSDGTMDM 600
VITDARTSLT RMAPILLGLD KGSHVLQPEV LHSKILAYKI IPKLGNGLFS VDGEKFPLEP 660
LQVEIMPRLC KTLLRNGRYV DTDFDSM 687 |
Gene Ontology | GO:0005794; C:Golgi apparatus; IDA:SGD. GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell. GO:0016020; C:membrane; IDA:SGD. GO:0005524; F:ATP binding; IEA:UniProtKB-KW. GO:0017050; F:D-erythro-sphingosine kinase activity; IDA:SGD. GO:0004143; F:diacylglycerol kinase activity; IEA:InterPro. GO:0008481; F:sphinganine kinase activity; IEA:EC. GO:0019722; P:calcium-mediated signaling; IMP:SGD. GO:0007205; P:protein kinase C-activating G-protein coupled receptor signaling pathway; IEA:InterPro. GO:0009408; P:response to heat; IDA:SGD. GO:0006665; P:sphingolipid metabolic process; IDA:SGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |