CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-027731
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 USP25 protein 
Protein Synonyms/Alias
 Ubiquitin carboxyl-terminal hydrolase 25 
Gene Name
 USP25 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
15LQQSAAQKHQQTFLNubiquitination[1]
57AVAFLTAKNAKTPQQubiquitination[1, 2]
60FLTAKNAKTPQQEETubiquitination[2, 3, 4]
99IDLTGDDKDDLQRAIubiquitination[2]
201RRLVLNYKPPSNAQDubiquitination[1, 2, 4, 5]
250SRAVEILKDAFKSNDubiquitination[1]
254EILKDAFKSNDSQQEubiquitination[4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 450 AA 
Protein Sequence
MTVEQNVLQQ SAAQKHQQTF LNQLREITGI NDTQILQQAL KDSNGNLELA VAFLTAKNAK 60
TPQQEETTYY QTALPGNDRY ISVGSQADTN VIDLTGDDKD DLQRAIALSL AESNRAFRET 120
GITDEEQAIS RVLEASIAEN KACLKRTPTE VWRDSRNPYD RKRQDKAPVG LKNVGNTCWF 180
SAVIQSLFNL LEFRRLVLNY KPPSNAQDLP RNQKEHRNLP FMRELRYLFA LLVGTKRKYV 240
DPSRAVEILK DAFKSNDSQQ EWHQDYRKFR ETTMYLIIGL ENFQRESYID SLLFLICAYQ 300
NNKELLSKGL YRGHDEELIS HYRRECLLKL NEQAAELFES GEDREVNNGL IIMNEFIVPF 360
LPLLLVDEME EKDILAVEDM RNRWCSYLGQ EMEPHLQEKL TDFLPKLLDC SMEIKSFHEP 420
PKLPSYSTHE LCERFARIML SLSRTPADGR 450 
Gene Ontology
 GO:0004221; F:ubiquitin thiolesterase activity; IEA:InterPro.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 
Interpro
 IPR018200; Pept_C19ubi-hydrolase_C_CS.
 IPR001394; Peptidase_C19.
 IPR009060; UBA-like.
 IPR003903; Ubiquitin-int_motif. 
Pfam
 PF00443; UCH
 PF02809; UIM 
SMART
 SM00726; UIM 
PROSITE
 PS00972; UCH_2_1
 PS50330; UIM 
PRINTS