UniProt Accession

 REN3B_HUMAN; Q9BZI7; D3DWI3; D3DWI4; Q0VAK8; Q9H1J0 

Genbank Protein ID

 AY013251; AF318576; CH471161; CH471161; CH471161; CH471161; BC121017 

Genbank Nucleotide ID

 AAG48511.1; AAG60691.1; EAW89842.1; EAW89844.1; EAW89845.1; EAW89846.1; AAI21018.1 

Protein Name

 Regulator of nonsense transcripts 3B 

Protein Synonyms/Alias

 Nonsense mRNA reducing factor 3B; Up-frameshift suppressor 3 homolog B; hUpf3B; Up-frameshift suppressor 3 homolog on chromosome X; hUpf3p-X 

Gene Name

 UPF3B 

Gene Synonyms/Alias

 RENT3B; UPF3X 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
173	SYATDNEKMTSTPET	ubiquitination	[1]
406	TLRDKGKKAESTESI	acetylation	[2]
421	GSSEKTEKKEEVVKR	acetylation	[2]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 

Functional Description

 Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2- UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF2 stimulates both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA upstream of exon-exon junctions. In vitro, stimulates translation; the function is indepenedent of association with UPF2 and components of the EJC core. 

Sequence Annotation

 REGION 30 255 Necessary for interaction with UPF2.
 REGION 52 57 Binds to UPF2.
 REGION 94 483 Sufficient for association with EJC core.
 REGION 430 447 Necessary for interaction with RBM8A and
 MOD_RES 169 169 Phosphothreonine.
 MOD_RES 198 198 Phosphothreonine.
 MOD_RES 310 310 Phosphoserine.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Disease mutation; Mental retardation; mRNA transport; Nonsense-mediated mRNA decay; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 483 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0035145; C:exon-exon junction complex; IDA:UniProtKB.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0003729; F:mRNA binding; IDA:UniProtKB.
 GO:0005487; F:nucleocytoplasmic transporter activity; NAS:UniProtKB.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0031124; P:mRNA 3'-end processing; TAS:Reactome.
 GO:0006406; P:mRNA export from nucleus; TAS:Reactome.
 GO:0000398; P:mRNA splicing, via spliceosome; TAS:Reactome.
 GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IDA:UniProtKB.
 GO:0045727; P:positive regulation of translation; IDA:UniProtKB.
 GO:0006369; P:termination of RNA polymerase II transcription; TAS:Reactome. 

Interpro

 IPR005120; Nonsense_mediated_decay_UPF3.
 IPR012677; Nucleotide-bd_a/b_plait. 

Pfam

 PF03467; Smg4_UPF3 

SMART

  

PROSITE

  

PRINTS