UniProt Accession

 IF4A_YEAST; P10081; D6VW46 

Genbank Protein ID

 X12813; X12814; X58099; X87371; Z49413; U25436; Z28284; BK006943; BK006944 

Genbank Nucleotide ID

 CAA31301.1; CAA31302.1; CAA41110.1; CAA60817.1; CAA89433.1; AAA91645.1; CAA82138.1; DAA08662.1; DAA09210.1 

Protein Name

 ATP-dependent RNA helicase eIF4A 

Protein Synonyms/Alias

 Eukaryotic initiation factor 4A; eIF-4A; Stimulator factor I 37 kDa component; Translation initiation factor 1/2; p37 

Gene Name

 TIF1; TIF2 

Gene Synonyms/Alias

 TIF41A; YKR059W; TIF41B; YJL138C; J0660 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
23	NYDKVVYKFDDMELD	acetylation	[1]
89	QRIDTSVKAPQALML	ubiquitination	[2]
226	PVRILVKKDELTLEG	acetylation	[1]
226	PVRILVKKDELTLEG	ubiquitination	[2]
272	IFCNTRRKVEELTTK	ubiquitination	[2]
279	KVEELTTKLRNDKFT	acetylation	[1]
284	TTKLRNDKFTVSAIY	acetylation	[1]
304	QERDTIMKEFRSGSS	acetylation	[1]
304	QERDTIMKEFRSGSS	ubiquitination	[2]
357	RGGRFGRKGVAINFV	ubiquitination	[2]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. 

Sequence Annotation

 DOMAIN 53 222 Helicase ATP-binding.
 DOMAIN 233 394 Helicase C-terminal.
 NP_BIND 66 73 ATP.
 MOTIF 22 50 Q motif.
 MOTIF 170 173 DEAD box.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 73 73 Phosphothreonine.
 MOD_RES 77 77 Phosphoserine.
 MOD_RES 129 129 Phosphoserine.
 MOD_RES 146 146 Phosphothreonine.  

Keyword

 3D-structure; Acetylation; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Helicase; Hydrolase; Initiation factor; Nucleotide-binding; Phosphoprotein; Protein biosynthesis; Reference proteome; RNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 395 AA 

Protein Sequence

Gene Ontology

 GO:0016281; C:eukaryotic translation initiation factor 4F complex; IGI:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0005840; C:ribosome; TAS:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004004; F:ATP-dependent RNA helicase activity; IDA:SGD.
 GO:0003743; F:translation initiation factor activity; IDA:SGD.
 GO:0006446; P:regulation of translational initiation; ISS:SGD. 

Interpro

 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000629; RNA-helicase_DEAD-box_CS.
 IPR014014; RNA_helicase_DEAD_Q_motif. 

Pfam

 PF00270; DEAD
 PF00271; Helicase_C 

SMART

 SM00487; DEXDc
 SM00490; HELICc 

PROSITE

 PS00039; DEAD_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51195; Q_MOTIF 

PRINTS