CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008198
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Dual specificity protein kinase CLK1 
Protein Synonyms/Alias
 CDC-like kinase 1 
Gene Name
 CLK1 
Gene Synonyms/Alias
 CLK 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
216LGEGAFGKVVECIDHubiquitination[1]
506MLEYDPAKRITLREAubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates: SRSF1, SRSF3 and PTPN1. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells and adenovirus E1A pre-mRNA. 
Sequence Annotation
 DOMAIN 161 477 Protein kinase.
 NP_BIND 167 175 ATP (By similarity).
 ACT_SITE 288 288 Proton acceptor (By similarity).
 BINDING 191 191 ATP (By similarity).
 MOD_RES 61 61 Phosphoserine.
 MOD_RES 138 138 Phosphothreonine.
 MOD_RES 140 140 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase; Tyrosine-protein kinase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 484 AA 
Protein Sequence
MAAGRRPASA LWPERRGSPL RGDLLGFQNV REPSSCGETL SGMRHSKRTY CPDWDDKDWD 60
YGKWRSSSSH KRRKRSHSSA QENKRCKYNH SKMCDSHYLE SRSINEKDYH SRRYIDEYRN 120
DYTQGCEPGH RQRDHESRYQ NHSSKSSGRS GRSSYKSKHR IHHSTSHRRS HGKSHRRKRT 180
RSVEDDEEGH LICQSGDVLS ARYEIVDTLG EGAFGKVVEC IDHKAGGRHV AVKIVKNVDR 240
YCEAARSEIQ VLEHLNTTDP NSTFRCVQML EWFEHHGHIC IVFELLGLST YDFIKENGFL 300
PFRLDHIRKM AYQICKSVNF LHSNKLTHTD LKPENILFVQ SDYTEAYNPK IKRDERTLIN 360
PDIKVVDFGS ATYDDEHHST LVSTRHYRAP EVILALGWSQ PCDVWSIGCI LIEYYLGFTV 420
FPTHDSKEHL AMMERILGPL PKHMIQKTRK RKYFHHDRLD WDEHSSAGRY VSRRCKPLKE 480
FMLSQDVEHE RLFDLIQKML EYDPAKRITL REALKHPFFD LLKKSI 526 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004715; F:non-membrane spanning protein tyrosine kinase activity; TAS:ProtInc.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
 GO:0004712; F:protein serine/threonine/tyrosine kinase activity; IEA:EC.
 GO:0008283; P:cell proliferation; TAS:ProtInc.
 GO:0018105; P:peptidyl-serine phosphorylation; IEA:Compara.
 GO:0018107; P:peptidyl-threonine phosphorylation; IEA:Compara.
 GO:0018108; P:peptidyl-tyrosine phosphorylation; IEA:Compara.
 GO:0046777; P:protein autophosphorylation; IEA:Compara.
 GO:0043484; P:regulation of RNA splicing; IMP:UniProtKB. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS