CPLM-006461

Genbank Nucleotide ID

General transcription factor IIF subunit 1

Protein Synonyms/Alias

General transcription factor IIF 74 kDa subunit; Transcription initiation factor IIF subunit alpha; TFIIF-alpha; Transcription initiation factor RAP74

Homo sapiens (Human)

Position	Peptide	Type	References
55	ERDLSNKKIYQEEEM	ubiquitination	[1, 2]
89	KKYGIVLKEFRPEDQ	ubiquitination	[2]
169	EEWERRNKVLNHFSI	ubiquitination	[2, 3, 4, 5]
183	IMQQRRLKDQDQDED	ubiquitination	[1]
407	TLRAAASKLEQGKRV	acetylation	[6]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[6] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]

Functional Description

TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.

MOD_RES 2 2 N-acetylalanine.
MOD_RES 217 217 Phosphoserine.
MOD_RES 218 218 Phosphoserine.
MOD_RES 221 221 Phosphoserine.
MOD_RES 224 224 Phosphoserine.
MOD_RES 331 331 Phosphothreonine.
MOD_RES 377 377 Phosphoserine.
MOD_RES 380 380 Phosphoserine.
MOD_RES 381 381 Phosphoserine.
MOD_RES 385 385 Phosphoserine.
MOD_RES 389 389 Phosphothreonine.
MOD_RES 391 391 Phosphoserine.
MOD_RES 407 407 N6-acetyllysine.
MOD_RES 431 431 Phosphoserine.
MOD_RES 433 433 Phosphoserine.
MOD_RES 436 436 Phosphoserine.
MOD_RES 446 446 Phosphothreonine.
MOD_RES 449 449 Phosphoserine.

3D-structure; Acetylation; Complete proteome; Direct protein sequencing; DNA-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0030054; C:cell junction; IDA:HPA.
GO:0005674; C:transcription factor TFIIF complex; TAS:ProtInc.
GO:0003824; F:catalytic activity; IEA:InterPro.
GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
GO:0019211; F:phosphatase activator activity; IDA:UniProtKB.
GO:0003713; F:transcription coactivator activity; TAS:ProtInc.
GO:0006370; P:7-methylguanosine mRNA capping; TAS:Reactome.
GO:0000398; P:mRNA splicing, via spliceosome; TAS:Reactome.
GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
GO:0050434; P:positive regulation of viral transcription; TAS:Reactome.
GO:0009615; P:response to virus; IEP:UniProtKB.
GO:0006368; P:transcription elongation from RNA polymerase II promoter; TAS:Reactome.
GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome.
GO:0022415; P:viral reproductive process; TAS:Reactome.

IPR008851; TFIIF-alpha.
IPR011039; TFIIF_interaction.
IPR011991; WHTH_DNA-bd_dom.

PF05793; TFIIF_alpha