UniProt Accession

 S19A1_HUMAN; P41440; B2R7U8; B7Z8C3; E9PFY4; O00553; O60227; Q13026; Q9BTX8 

Genbank Protein ID

 U15939; U19720; S78996; U17566; U92873; U92869; U92870; U92871; U92872; AF004354; AK303168; AK313125; AL163302; BX322561; CH471079; BC003068 

Genbank Nucleotide ID

 AAA98442.1; AAC50180.1; AAB35058.1; AAA74914.1; AAC26162.1; AAC26162.1; AAC26162.1; AAC26162.1; AAC26162.1; AAB61417.1; BAH13909.1; BAG35945.1; CAB90483.1; EAX09331.1; AAH03068.1 

Protein Name

 Folate transporter 1 

Protein Synonyms/Alias

 FOLT; Intestinal folate carrier 1; IFC-1; Placental folate transporter; Reduced folate carrier protein; RFC; Solute carrier family 19 member 1 

Gene Name

 SLC19A1 

Gene Synonyms/Alias

 FLOT1; RFC1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
10	PSSPAVEKQVPVEPG	ubiquitination	[1, 2]
237	MNPGPGGKLGHALRV	ubiquitination	[1, 2, 3, 4, 5]
479	LRSAAEEKAAQALSV	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8, 9, 10]
489	QALSVQDKGLGGLQP	ubiquitination	[1, 2, 6]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [8] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [9] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [10] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Transporter for the intake of folate. Uptake of folate in human placental choriocarcinoma cells occurs by a novel mechanism called potocytosis which functionally couples three components, namely the folate receptor, the folate transporter, and a V-type H(+)-pump. 

Sequence Annotation

 MOD_RES 485 485 Phosphoserine (By similarity).
 MOD_RES 499 499 Phosphoserine.
 MOD_RES 503 503 Phosphoserine.
 CARBOHYD 58 58 N-linked (GlcNAc...) (Potential).  

Keyword

 Alternative splicing; Complete proteome; Folate-binding; Glycoprotein; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 591 AA 

Protein Sequence

Gene Ontology

 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0005542; F:folic acid binding; IEA:UniProtKB-KW.
 GO:0008517; F:folic acid transporter activity; TAS:ProtInc.
 GO:0015350; F:methotrexate transporter activity; TAS:ProtInc.
 GO:0008518; F:reduced folate carrier activity; IEA:InterPro.
 GO:0046655; P:folic acid metabolic process; TAS:Reactome. 

Interpro

 IPR002666; Folate_carrier.
 IPR016196; MFS_dom_general_subst_transpt. 

Pfam

 PF01770; Folate_carrier 

SMART

  

PROSITE

  

PRINTS