CPLM-009696

Genbank Nucleotide ID

Ubiquitin-conjugating enzyme E2 B

Protein Synonyms/Alias

RAD6 homolog B; HR6B; Ubiquitin carrier protein B; Ubiquitin-conjugating enzyme E2(14k); Ubiquitin-protein ligase B

Rattus norvegicus (Rat)

Position	Peptide	Type	References
66	FSEEYPNKPPTVRFL	acetylation	[1]

[1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]

Functional Description

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation (By similarity). In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV- damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B- RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.

ACT_SITE 88 88 Glycyl thioester intermediate (By

ATP-binding; Cell membrane; Complete proteome; DNA damage; DNA repair; Ligase; Membrane; Nucleotide-binding; Nucleus; Reference proteome; Ubl conjugation pathway.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IEA:Compara.
GO:0033503; C:HULC complex; ISS:UniProtKB.
GO:0000790; C:nuclear chromatin; IEA:Compara.
GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
GO:0005657; C:replication fork; IEA:Compara.
GO:0001741; C:XY body; IEA:Compara.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO:0004842; F:ubiquitin-protein ligase activity; ISS:UniProtKB.
GO:0060070; P:canonical Wnt receptor signaling pathway; IEA:Compara.
GO:0032869; P:cellular response to insulin stimulus; IEP:RGD.
GO:0051026; P:chiasma assembly; IEA:Compara.
GO:0033522; P:histone H2A ubiquitination; IEA:Compara.
GO:0070076; P:histone lysine demethylation; IEA:Compara.
GO:0001701; P:in utero embryonic development; IEA:Compara.
GO:0006344; P:maintenance of chromatin silencing; IEA:Compara.
GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
GO:0043951; P:negative regulation of cAMP-mediated signaling; IEA:Compara.
GO:0033128; P:negative regulation of histone phosphorylation; IEA:Compara.
GO:0010845; P:positive regulation of reciprocal meiotic recombination; IEA:Compara.
GO:0006301; P:postreplication repair; IEA:Compara.
GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IEA:Compara.
GO:0051865; P:protein autoubiquitination; IEA:Compara.
GO:0070979; P:protein K11-linked ubiquitination; ISS:UniProtKB.
GO:0070936; P:protein K48-linked ubiquitination; ISS:UniProtKB.
GO:0070534; P:protein K63-linked ubiquitination; ISS:UniProtKB.
GO:0006513; P:protein monoubiquitination; IEA:Compara.
GO:0050821; P:protein stabilization; IEA:Compara.
GO:0042493; P:response to drug; IEA:Compara.
GO:0001666; P:response to hypoxia; IEP:RGD.
GO:0009411; P:response to UV; IEA:Compara.
GO:0007288; P:sperm axoneme assembly; IEA:Compara.
GO:0070193; P:synaptonemal complex organization; IEA:Compara.
GO:0006511; P:ubiquitin-dependent protein catabolic process; IEP:RGD.

IPR000608; UBQ-conjugat_E2.
IPR023313; UBQ-conjugating_AS.
IPR016135; UBQ-conjugating_enzyme/RWD.

PS00183; UBIQUITIN_CONJUGAT_1
PS50127; UBIQUITIN_CONJUGAT_2