CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005247
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protease HtpX 
Protein Synonyms/Alias
 Heat shock protein HtpX 
Gene Name
 htpX 
Gene Synonyms/Alias
 b1829; JW1818 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
231HADAGSAKLVGREKMacetylation[1]
281MTHPPLDKRIEALRTacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Membrane-localized protease able to endoproteolytically degrade overproduced SecY but not YccA, another membrane protein. It seems to cleave SecY at specific cytoplasmic sites. Does not require ATP. Its natural substrate has not been identified. Probably plays a role in the quality control of integral membrane proteins. 
Sequence Annotation
 ACT_SITE 140 140 By similarity.
 METAL 139 139 Zinc; catalytic (By similarity).
 METAL 143 143 Zinc; catalytic (By similarity).
 METAL 222 222 Zinc; catalytic (By similarity).  
Keyword
 Autocatalytic cleavage; Cell inner membrane; Cell membrane; Complete proteome; Hydrolase; Membrane; Metal-binding; Metalloprotease; Protease; Reference proteome; Stress response; Transmembrane; Transmembrane helix; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 293 AA 
Protein Sequence
MMRIALFLLT NLAVMVVFGL VLSLTGIQSS SVQGLMIMAL LFGFGGSFVS LLMSKWMALR 60
SVGGEVIEQP RNERERWLVN TVATQARQAG IAMPQVAIYH APDINAFATG ARRDASLVAV 120
STGLLQNMSP DEAEAVIAHE ISHIANGDMV TMTLIQGVVN TFVIFISRIL AQLAAGFMGG 180
NRDEGEESNG NPLIYFAVAT VLELVFGILA SIITMWFSRH REFHADAGSA KLVGREKMIA 240
ALQRLKTSYE PQEATSMMAL CINGKSKSLS ELFMTHPPLD KRIEALRTGE YLK 293 
Gene Ontology
 GO:0016021; C:integral to membrane; IDA:EcoCyc.
 GO:0005886; C:plasma membrane; IDA:EcoliWiki.
 GO:0004222; F:metalloendopeptidase activity; IDA:EcoCyc.
 GO:0008270; F:zinc ion binding; IDA:EcoCyc.
 GO:0006508; P:proteolysis; IDA:EcoCyc.
 GO:0006950; P:response to stress; IEA:UniProtKB-KW.
 GO:0009266; P:response to temperature stimulus; IEP:EcoCyc. 
Interpro
 IPR022919; Pept_M48_protease_HtpX.
 IPR001915; Peptidase_M48. 
Pfam
 PF01435; Peptidase_M48 
SMART
  
PROSITE
 PS00142; ZINC_PROTEASE 
PRINTS