UniProt Accession

 DBP2_YEAST; P24783; D6W169; Q05456 

Genbank Protein ID

 X52649; Z71388; Z69382; M64991; BK006947 

Genbank Nucleotide ID

 CAA36874.1; CAA95991.1; CAA93395.1; DAA10435.1 

Protein Name

 ATP-dependent RNA helicase DBP2 

Protein Synonyms/Alias

 DEAD box protein 2; p68-like protein 

Gene Name

 DBP2 

Gene Synonyms/Alias

 YNL112W; N1945 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
12	GRDQQYNKTNYKSRG	acetylation	[1]
60	NQPQELIKPNWDEEL	ubiquitination	[2]
226	CVYGGVPKSQQIRDL	ubiquitination	[2]
283	GFEPQIRKIVDQIRP	acetylation	[1]
350	EKRDRLNKYLETASQ	acetylation	[1]
474	QNKGLGAKLISIMRE	ubiquitination	[2, 3]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

 ATP-dependent RNA helicase involved nonsense-mediated mRNA decay and ribosome biogenesis through rRNA processing. 

Sequence Annotation

 DOMAIN 144 319 Helicase ATP-binding.
 DOMAIN 347 494 Helicase C-terminal.
 NP_BIND 157 164 ATP.
 REGION 505 530 RNA-binding RGG-box.
 MOTIF 113 141 Q motif.
 MOTIF 267 270 DEAD box.
 MOD_RES 88 88 Phosphoserine.
 MOD_RES 90 90 Phosphoserine.  

Keyword

 ATP-binding; Complete proteome; Cytoplasm; Helicase; Hydrolase; Nonsense-mediated mRNA decay; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Ribosome biogenesis; RNA-binding; rRNA processing. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 546 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0003724; F:RNA helicase activity; ISS:SGD.
 GO:0008186; F:RNA-dependent ATPase activity; IMP:SGD.
 GO:0071042; P:nuclear polyadenylation-dependent mRNA catabolic process; IMP:SGD.
 GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IPI:SGD.
 GO:0006364; P:rRNA processing; IMP:SGD. 

Interpro

 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000629; RNA-helicase_DEAD-box_CS.
 IPR014014; RNA_helicase_DEAD_Q_motif. 

Pfam

 PF00270; DEAD
 PF00271; Helicase_C 

SMART

 SM00487; DEXDc
 SM00490; HELICc 

PROSITE

 PS00039; DEAD_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51195; Q_MOTIF 

PRINTS