UniProt Accession

 G6PI_ECOLI; P0A6T1; P11537; Q2M6S9 

Genbank Protein ID

 X15196; U00006; U00096; AP009048 

Genbank Nucleotide ID

 CAA33268.1; AAC43119.1; AAC76995.1; BAE78027.1 

Protein Name

 Glucose-6-phosphate isomerase 

Protein Synonyms/Alias

 GPI; Phosphoglucose isomerase; PGI; Phosphohexose isomerase; PHI 

Gene Name

 pgi 

Gene Synonyms/Alias

 b4025; JW3985 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
2	******MKNINPTQT	acetylation	[1, 2]
17	AAWQALQKHFDEMKD	acetylation	[2]
23	QKHFDEMKDVTIADL	acetylation	[1, 2]
33	TIADLFAKDGDRFSK	acetylation	[2]
65	ITEETLAKLQDLAKE	acetylation	[1, 2]
71	AKLQDLAKECDLAGA	acetylation	[1, 2]
80	CDLAGAIKSMFSGEK	acetylation	[1, 2, 3]
87	KSMFSGEKINRTENR	acetylation	[2]
114	TPILVDGKDVMPEVN	acetylation	[2]
126	EVNAVLEKMKTFSEA	acetylation	[1, 2]
128	NAVLEKMKTFSEAII	acetylation	[2]
140	AIISGEWKGYTGKAI	acetylation	[2]
194	THIAEVLKKVNPETT	acetylation	[2]
195	HIAEVLKKVNPETTL	acetylation	[2]
208	TLFLVASKTFTTQET	acetylation	[2]
228	SARDWFLKAAGDEKH	acetylation	[2, 3]
234	LKAAGDEKHVAKHFA	acetylation	[1, 2, 3]
238	GDEKHVAKHFAALST	acetylation	[1, 2]
435	TEALAFGKSREVVEQ	acetylation	[2]
449	QEYRDQGKDPATLDY	acetylation	[1, 2, 4]
461	LDYVVPFKVFEGNRP	acetylation	[2]
525	NRILPELKDDKEISS	acetylation	[2]
528	LPELKDDKEISSHDS	acetylation	[2]

Reference

 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [3] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
 Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y.
 Mol Cell Proteomics. 2009 Feb;8(2):215-25. [PMID: 18723842]
 [4] The diversity of lysine-acetylated proteins in Escherichia coli.
 Yu BJ, Kim JA, Moon JH, Ryu SE, Pan JG.
 J Microbiol Biotechnol. 2008 Sep;18(9):1529-36. [PMID: 18852508] 

Functional Description

  

Sequence Annotation

 ACT_SITE 355 355 Proton donor (By similarity).
 ACT_SITE 386 386 By similarity.
 ACT_SITE 514 514 By similarity.
 MOD_RES 80 80 N6-acetyllysine.
 MOD_RES 228 228 N6-acetyllysine.
 MOD_RES 234 234 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Cytoplasm; Gluconeogenesis; Glycolysis; Isomerase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 549 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0004347; F:glucose-6-phosphate isomerase activity; IDA:EcoCyc.
 GO:0034599; P:cellular response to oxidative stress; IMP:EcoCyc.
 GO:0006094; P:gluconeogenesis; IEA:HAMAP.
 GO:0006096; P:glycolysis; IMP:EcoCyc. 

Interpro

 IPR001672; G6P_Isomerase.
 IPR023096; G6P_Isomerase_C.
 IPR018189; Phosphoglucose_isomerase_CS. 

Pfam

 PF00342; PGI 

SMART

  

PROSITE

 PS00765; P_GLUCOSE_ISOMERASE_1
 PS00174; P_GLUCOSE_ISOMERASE_2
 PS51463; P_GLUCOSE_ISOMERASE_3 

PRINTS

 PR00662; G6PISOMERASE.