CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030635
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Dedicator of cytokinesis protein 2 
Protein Synonyms/Alias
 cDNA FLJ46228 fis, clone TESTI4014908, highly similar to Dedicator of cytokinesis protein 2 
Gene Name
 DOCK2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
67VFLRAINKFAETMNQubiquitination[1]
115FSHAKYNKILNKYGDubiquitination[1, 2, 3]
119KYNKILNKYGDMRRLubiquitination[1, 3]
137SIRDMWYKLGQNKICubiquitination[1, 4]
188QRSGDFKKFENEIILubiquitination[1, 3, 4]
240ENFVNLVKGLLEKLLubiquitination[1]
245LVKGLLEKLLDYRGVubiquitination[1, 4]
258GVMTDESKDNRMSCTubiquitination[3]
420FPSFLRNKVFIYRGKubiquitination[1, 3]
460SAPGDDVKNAPGQYIubiquitination[1]
483LDEHPRFKNKPVPDQubiquitination[1]
485EHPRFKNKPVPDQIIubiquitination[1, 3, 4]
496DQIINFYKSNYVQRFubiquitination[1, 3, 4]
535TSFVTAYKLPGILRWubiquitination[1, 4]
609GGFAKYEKAFFTEEYubiquitination[1, 3]
668DRMEECFKNLKMKVEubiquitination[1, 3]
671EECFKNLKMKVEKEYubiquitination[1]
673CFKNLKMKVEKEYGVubiquitination[1]
676NLKMKVEKEYGVREMubiquitination[1]
755GSTLPEVKLRRSKKRubiquitination[1]
774SVVFADEKAAAESDLubiquitination[1, 3]
787DLKRLSRKHEFMSDTubiquitination[1]
856LQLSDGDKKTLTRKKubiquitination[1]
857QLSDGDKKTLTRKKVubiquitination[1, 3]
862DKKTLTRKKVNQFFKubiquitination[1]
863KKTLTRKKVNQFFKTubiquitination[1]
869KKVNQFFKTMLASKSubiquitination[4]
875FKTMLASKSAEEGKQubiquitination[1, 3]
881SKSAEEGKQIPDSLSubiquitination[1, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 891 AA 
Protein Sequence
MTAILNQMGD QHYSFYIETF QTSSELVDFL METFIMFKDL IGKNVYPGDW MAMSMVQNRV 60
FLRAINKFAE TMNQKFLEHT NFEFQLWNNY FHLAVAFITQ DSLQLEQFSH AKYNKILNKY 120
GDMRRLIGFS IRDMWYKLGQ NKICFIPGMV GPILEMTLIP EAELRKATIP IFFDMMLCEY 180
QRSGDFKKFE NEIILKLDHE VEGGRGDEQY MQLLESILME CAAEHPTIAK SVENFVNLVK 240
GLLEKLLDYR GVMTDESKDN RMSCTVNLLN FYKDNNREEM YIRYLYKLRD LHLDCDNYTE 300
AAYTLLLHTW LLKWSDEQCA SQVMQTGQQH PQTHRQLKET LYETIIGYFD KGKMWEEAIS 360
LCKELAEQYE MEIFDYELLS QNLIQQAKFY ESIMKILRPK PDYFAVGYYG QGFPSFLRNK 420
VFIYRGKEYE RREDFQMQLM TQFPNAEKMN TTSAPGDDVK NAPGQYIQCF TVQPVLDEHP 480
RFKNKPVPDQ IINFYKSNYV QRFHYSRPVR RGTVDPENEF ASMWIERTSF VTAYKLPGIL 540
RWFEVVHMSQ TTISPLENAI ETMSTANEKI LMMINQYQSD ETLPINPLSM LLNGIVDPAV 600
MGGFAKYEKA FFTEEYVRDH PEDQDKLTHL KDLIAWQIPF LGAGIKIHEK RVSDNLRPFH 660
DRMEECFKNL KMKVEKEYGV REMPDFDDRR VGRPRSMLRS YRQMSIISLA SMNSDCSTPS 720
KPTSESFDLE LASPKTPRVE QEEPISPGST LPEVKLRRSK KRTKRSSVVF ADEKAAAESD 780
LKRLSRKHEF MSDTNLSEHA AIPLKASVLS QMSFASQSMP TIPALALSVA GIPGLDEANT 840
SPRLSQTFLQ LSDGDKKTLT RKKVNQFFKT MLASKSAEEG KQIPDSLSTD L 891 
Gene Ontology
 GO:0009055; F:electron carrier activity; IEA:InterPro.
 GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:InterPro.
 GO:0020037; F:heme binding; IEA:InterPro.
 GO:0030675; F:Rac GTPase activator activity; IEA:Compara.
 GO:0046633; P:alpha-beta T cell proliferation; IEA:Compara.
 GO:0006935; P:chemotaxis; IEA:Compara.
 GO:0007010; P:cytoskeleton organization; IEA:Compara.
 GO:0001768; P:establishment of T cell polarity; IEA:Compara.
 GO:0001771; P:immunological synapse formation; IEA:Compara.
 GO:0044351; P:macropinocytosis; IEA:Compara.
 GO:0001766; P:membrane raft polarization; IEA:Compara.
 GO:0002277; P:myeloid dendritic cell activation involved in immune response; IEA:Compara.
 GO:0045060; P:negative thymic T cell selection; IEA:Compara.
 GO:0050766; P:positive regulation of phagocytosis; IEA:Compara.
 GO:0045059; P:positive thymic T cell selection; IEA:Compara.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. 
Interpro
 IPR009056; Cyt_c_dom.
 IPR027357; DHR-2.
 IPR026791; DOCK.
 IPR026799; DOCK_A.
 IPR010703; DOCK_C. 
Pfam
 PF06920; Ded_cyto 
SMART
  
PROSITE
 PS51651; DHR_2 
PRINTS