UniProt Accession

 THTM_HUMAN; P25325; O75750 

Genbank Protein ID

 X59434; CR456523; BT019636; Z73420; BC003508; BC016737; BC018717 

Genbank Nucleotide ID

 CAA42060.1; CAG30409.1; AAV38442.1; CAA97763.1; AAH03508.1; AAH16737.1; AAH18717.1 

Protein Name

 3-mercaptopyruvate sulfurtransferase 

Protein Synonyms/Alias

 MST 

Gene Name

 MPST 

Gene Synonyms/Alias

 TST2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
40	DASWYLPKLGRDARR	ubiquitination	[1]
146	NLPLSSGKSQPAPAE	ubiquitination	[1]
164	QLDPAFIKTYEDIKE	ubiquitination	[1, 2]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Detoxifies cyanide and is required for thiosulfate biosynthesis. Acts as an antioxidant. In combination with cysteine aminotransferase (CAT), contributes to the catabolism of cysteine and is an important producer of hydrogen sulfide in the brain, retina and vascular endothelial cells. Hydrogen sulfide H(2)S is an important synaptic modulator, signaling molecule, smooth muscle contractor and neuroprotectant. Its production by the 3MST/CAT pathway is regulated by calcium ions (By similarity). 

Sequence Annotation

 DOMAIN 25 144 Rhodanese 1.
 DOMAIN 174 288 Rhodanese 2.
 REGION 145 160 Hinge.
 ACT_SITE 248 248 Cysteine persulfide intermediate (By
 BINDING 188 188 Substrate (By similarity).
 DISULFID 264 264 Interchain (with C-264); redox-active (By  

Keyword

 3D-structure; Cell junction; Complete proteome; Cytoplasm; Direct protein sequencing; Disulfide bond; Mitochondrion; Redox-active center; Reference proteome; Repeat; Synapse; Synaptosome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 297 AA 

Protein Sequence

Gene Ontology

 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0043005; C:neuron projection; ISS:UniProtKB.
 GO:0045202; C:synapse; IEA:UniProtKB-KW.
 GO:0016784; F:3-mercaptopyruvate sulfurtransferase activity; IEA:EC.
 GO:0004792; F:thiosulfate sulfurtransferase activity; TAS:ProtInc.
 GO:0009440; P:cyanate catabolic process; TAS:ProtInc.
 GO:0070814; P:hydrogen sulfide biosynthetic process; ISS:UniProtKB.
 GO:0009636; P:response to toxic substance; TAS:ProtInc. 

Interpro

 IPR001763; Rhodanese-like_dom.
 IPR001307; Thiosulphate_STrfase_CS. 

Pfam

 PF00581; Rhodanese 

SMART

 SM00450; RHOD 

PROSITE

 PS00380; RHODANESE_1
 PS00683; RHODANESE_2
 PS50206; RHODANESE_3 

PRINTS