CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005023
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA polymerase epsilon catalytic subunit A 
Protein Synonyms/Alias
 DNA polymerase II subunit A 
Gene Name
 POL2 
Gene Synonyms/Alias
 DUN2; YNL262W; N0825 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
22ARYSAGNKYNTLSNNacetylation[1]
257HVRVSIDKDIRVGKWubiquitination[2]
1063LKEYEGQKSTSITTAubiquitination[3]
1198FKQTSLTKFFSKTKNacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] A perturbed ubiquitin landscape distinguishes between ubiquitin in trafficking and in proteolysis.
 Ziv I, Matiuhin Y, Kirkpatrick DS, Erpapazoglou Z, Leon S, Pantazopoulou M, Kim W, Gygi SP, Haguenauer-Tsapis R, Reis N, Glickman MH, Kleifeld O.
 Mol Cell Proteomics. 2011 May;10(5):M111.009753. [PMID: 21427232]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 DNA polymerase epsilon (DNA polymerase II) participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair. 
Sequence Annotation
 ZN_FING 2108 2133 CysA-type.
 MOTIF 2164 2181 CysB motif.
 METAL 2108 2108 Zinc (By similarity).
 METAL 2111 2111 Zinc (By similarity).
 METAL 2130 2130 Zinc (By similarity).
 METAL 2133 2133 Zinc (By similarity).
 METAL 2164 2164 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 2167 2167 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 2179 2179 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 2181 2181 Iron-sulfur (4Fe-4S) (By similarity).  
Keyword
 4Fe-4S; Complete proteome; Direct protein sequencing; DNA replication; DNA-binding; DNA-directed DNA polymerase; Iron; Iron-sulfur; Metal-binding; Nucleotidyltransferase; Nucleus; Reference proteome; Transferase; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2222 AA 
Protein Sequence
MMFGKKKNNG GSSTARYSAG NKYNTLSNNY ALSAQQLLNA SKIDDIDSMM GFERYVPPQY 60
NGRFDAKDID QIPGRVGWLT NMHATLVSQE TLSSGSNGGG NSNDGERVTT NQGISGVDFY 120
FLDEEGGSFK STVVYDPYFF IACNDESRVN DVEELVKKYL ESCLKSLQII RKEDLTMDNH 180
LLGLQKTLIK LSFVNSNQLF EARKLLRPIL QDNANNNVQR NIYNVAANGS EKVDAKHLIE 240
DIREYDVPYH VRVSIDKDIR VGKWYKVTQQ GFIEDTRKIA FADPVVMAFD IETTKPPLKF 300
PDSAVDQIMM ISYMIDGEGF LITNREIISE DIEDFEYTPK PEYPGFFTIF NENDEVALLQ 360
RFFEHIRDVR PTVISTFNGD FFDWPFIHNR SKIHGLDMFD EIGFAPDAEG EYKSSYCSHM 420
DCFRWVKRDS YLPQGSQGLK AVTQSKLGYN PIELDPELMT PYAFEKPQHL SEYSVSDAVA 480
TYYLYMKYVH PFIFSLCTII PLNPDETLRK GTGTLCEMLL MVQAYQHNIL LPNKHTDPIE 540
RFYDGHLLES ETYVGGHVES LEAGVFRSDL KNEFKIDPSA IDELLQELPE ALKFSVEVEN 600
KSSVDKVTNF EEIKNQITQK LLELKENNIR NELPLIYHVD VASMYPNIMT TNRLQPDSIK 660
AERDCASCDF NRPGKTCARK LKWAWRGEFF PSKMDEYNMI KRALQNETFP NKNKFSKKKV 720
LTFDELSYAD QVIHIKKRLT EYSRKVYHRV KVSEIVEREA IVCQRENPFY VDTVKSFRDR 780
RYEFKGLAKT WKGNLSKIDP SDKHARDEAK KMIVLYDSLQ LAHKVILNSF YGYVMRKGSR 840
WYSMEMAGIT CLTGATIIQM ARALVERVGR PLELDTDGIW CILPKSFPET YFFTLENGKK 900
LYLSYPCSML NYRVHQKFTN HQYQELKDPL NYIYETHSEN TIFFEVDGPY KAMILPSSKE 960
EGKGIKKRYA VFNEDGSLAE LKGFELKRRG ELQLIKNFQS DIFKVFLEGD TLEGCYSAVA 1020
SVCNRWLDVL DSHGLMLEDE DLVSLICENR SMSKTLKEYE GQKSTSITTA RRLGDFLGED 1080
MVKDKGLQCK YIISSKPFNA PVTERAIPVA IFSADIPIKR SFLRRWTLDP SLEDLDIRTI 1140
IDWGYYRERL GSAIQKIITI PAALQGVSNP VPRVEHPDWL KRKIATKEDK FKQTSLTKFF 1200
SKTKNVPTMG KIKDIEDLFE PTVEEDNAKI KIARTTKKKA VSKRKRNQLT NEEDPLVLPS 1260
EIPSMDEDYV GWLNYQKIKW KIQARDRKRR DQLFGNTNSS RERSALGSMI RKQAESYANS 1320
TWEVLQYKDS GEPGVLEVFV TINGKVQNIT FHIPKTIYMK FKSQTMPLQK IKNCLIEKSS 1380
ASLPNNPKTS NPAGGQLFKI TLPESVFLEE KENCTSIFND ENVLGVFEGT ITPHQRAIMD 1440
LGASVTFRSK AMGALGKGIQ QGFEMKDLSM AENERYLSGF SMDIGYLLHF PTSIGYEFFS 1500
LFKSWGDTIT ILVLKPSNQA QEINASSLGQ IYKQMFEKKK GKIETYSYLV DIKEDINFEF 1560
VYFTDISKLY RRLSQETTKL KEERGLQFLL LLQSPFITKL LGTIRLLNQM PIVKLSLNEV 1620
LLPQLNWQPT LLKKLVNHVL SSGSWISHLI KLSQYSNIPI CNLRLDSMDY IIDVLYARKL 1680
KKENIVLWWN EKAPLPDHGG IQNDFDLNTS WIMNDSEFPK INNSGVYDNV VLDVGVDNLT 1740
VNTILTSALI NDAEGSDLVN NNMGIDDKDA VINSPSEFVH DAFSNDALNV LRGMLKEWWD 1800
EALKENSTAD LLVNSLASWV QNPNAKLFDG LLRYHVHNLT KKALLQLVNE FSALGSTIVY 1860
ADRNQILIKT NKYSPENCYA YSQYMMKAVR TNPMFSYLDL NIKRYWDLLI WMDKFNFSGL 1920
ACIEIEEKEN QDYTAVSQWQ LKKFLSPIYQ PEFEDWMMII LDSMLKTKQS YLKLNSGTQR 1980
PTQIVNVKKQ DKEDSVENSL NGFSHLFSKP LMKRVKKLFK NQQEFILDPQ YEADYVIPVL 2040
PGSHLNVKNP LLELVKSLCH VMLLSKSTIL EIRTLRKELL KIFELREFAK VAEFKDPSLS 2100
LVVPDFLCEY CFFISDIDFC KAAPESIFSC VRCHKAFNQV LLQEHLIQKL RSDIESYLIQ 2160
DLRCSRCHKV KRDYMSAHCP CAGAWEGTLP RESIVQKLNV FKQVAKYYGF DILLSCIADL 2220
TI 2222 
Gene Ontology
 GO:0008622; C:epsilon DNA polymerase complex; IDA:SGD.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0003887; F:DNA-directed DNA polymerase activity; IDA:SGD.
 GO:0003690; F:double-stranded DNA binding; IDA:SGD.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003697; F:single-stranded DNA binding; IDA:SGD.
 GO:0008310; F:single-stranded DNA specific 3'-5' exodeoxyribonuclease activity; IMP:SGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006284; P:base-excision repair; IMP:SGD.
 GO:0045004; P:DNA replication proofreading; IMP:SGD.
 GO:0000731; P:DNA synthesis involved in DNA repair; IMP:SGD.
 GO:0006303; P:double-strand break repair via nonhomologous end joining; IMP:SGD.
 GO:0042276; P:error-prone translesion synthesis; IDA:SGD.
 GO:0035822; P:gene conversion; IMP:SGD.
 GO:0070868; P:heterochromatin organization involved in chromatin silencing; IMP:SGD.
 GO:0031573; P:intra-S DNA damage checkpoint; IMP:SGD.
 GO:0006272; P:leading strand elongation; IMP:SGD.
 GO:0033314; P:mitotic DNA replication checkpoint; IMP:SGD.
 GO:0007064; P:mitotic sister chromatid cohesion; IMP:SGD.
 GO:0006297; P:nucleotide-excision repair, DNA gap filling; IMP:SGD. 
Interpro
 IPR006172; DNA-dir_DNA_pol_B.
 IPR006133; DNA-dir_DNA_pol_B_exonuc.
 IPR006134; DNA-dir_DNA_pol_B_multi_dom.
 IPR013697; DNA_pol_e_suA_C.
 IPR012337; RNaseH-like_dom. 
Pfam
 PF00136; DNA_pol_B
 PF03104; DNA_pol_B_exo1
 PF08490; DUF1744 
SMART
 SM00486; POLBc 
PROSITE
 PS00116; DNA_POLYMERASE_B 
PRINTS