CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002304
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Myosin light chain 1/3, skeletal muscle isoform 
Protein Synonyms/Alias
 MLC1/MLC3; MLC1F/MLC3F; Myosin light chain alkali 1/2; Myosin light chain A1/A2 
Gene Name
 MYL1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
141EGLRVFDKEGNGTVMacetylation[1, 2]
141EGLRVFDKEGNGTVMubiquitination[3, 4, 5]
Reference
 [1] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Regulatory light chain of myosin. Does not bind calcium. 
Sequence Annotation
 DOMAIN 50 85 EF-hand 1.
 DOMAIN 127 162 EF-hand 2.
 DOMAIN 162 194 EF-hand 3.
 MOD_RES 2 2 N,N,N-trimethylalanine (By similarity).  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Methylation; Motor protein; Muscle protein; Myosin; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 194 AA 
Protein Sequence
MAPKKDVKKP VAAAAAAPAP APAPAPAPAP AKPKEEKIDL SAIKIEFSKE QQDEFKEAFL 60
LFDRTGDSKI TLSQVGDVLR ALGTNPTNAE VRKVLGNPSN EELNAKKIEF EQFLPMMQAI 120
SNNKDQATYE DFVEGLRVFD KEGNGTVMGA ELRHVLATLG EKMKEEEVEA LMAGQEDSNG 180
CINYEAFVKH IMSI 194 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005859; C:muscle myosin complex; NAS:BHF-UCL.
 GO:0030017; C:sarcomere; NAS:BHF-UCL.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0008307; F:structural constituent of muscle; NAS:BHF-UCL.
 GO:0060048; P:cardiac muscle contraction; IEA:Compara.
 GO:0030049; P:muscle filament sliding; NAS:BHF-UCL.
 GO:0007517; P:muscle organ development; NAS:UniProtKB. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR002048; EF_hand_dom. 
Pfam
 PF13405; EF_hand_4 
SMART
 SM00054; EFh 
PROSITE
 PS50222; EF_HAND_2 
PRINTS