| Tag | Content |
|---|
| CPLM ID | CPLM-023424 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Flap endonuclease GEN |
Protein Synonyms/Alias | Flap structure-specific endonuclease GEN; Xpg-like endonuclease; DmGEN |
Gene Name | Gen |
Gene Synonyms/Alias | CG10670 |
Created Date | July 27, 2013 |
Organism | Drosophila melanogaster (Fruit fly) |
NCBI Taxa ID | 7227 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 360 | WRQPNLVKFIKQIGH | acetylation | [1] |
|
Reference | [1] Proteome-wide mapping of the Drosophila acetylome demonstrates a high degree of conservation of lysine acetylation. Weinert BT, Wagner SA, Horn H, Henriksen P, Liu WR, Olsen JV, Jensen LJ, Choudhary C. Sci Signal. 2011 Jul 26;4(183):ra48. [ PMID: 21791702] |
Functional Description | Endonuclease which cleaves flap structures at the junction between single-stranded DNA and double-stranded DNA. Specific for 5'-overhanging flap structures in which the 5'- upstream of the flap is completely double-stranded. Prefers the blocked-flap structures similar to those occurring at replication forks, in which the 5' single-strand overhang of the flap is double-stranded. Also possesses weak 5'- to 3'-exonuclease activity on nicked but not gapped double-stranded DNA. Does not cleave bubble-like or Holliday junction substrates. |
Sequence Annotation | REGION 1 90 N-domain.
REGION 131 227 I-domain.
METAL 30 30 Magnesium 1 (By similarity).
METAL 74 74 Magnesium 1 (By similarity).
METAL 143 143 Magnesium 1 (By similarity).
METAL 145 145 Magnesium 1 (Probable).
METAL 164 164 Magnesium 2 (By similarity).
METAL 166 166 Magnesium 2 (By similarity).
METAL 223 223 Magnesium 2 (By similarity).
MOD_RES 439 439 Phosphotyrosine. |
Keyword | Complete proteome; DNA damage; DNA repair; Endonuclease; Exonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease; Nucleus; Phosphoprotein; Reference proteome. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 726 AA |
Protein Sequence | MGVKELWGVL TPHCERKPIN ELRGKKVAID LAGWVCESLN VVDYFVHPRH HLKNLFFRTC 60
YLIWEQVTPV FVLEGVAPKL KSQVIAKRNE LQFRGVKPKN SPECTQSQPS KGDKGRSRFN 120
HVLKQCETLL LSMGIQCVQG PGEAEAYCAF LNKHGLVDGV ISQDSDCFAY GAVRVYRNFS 180
VSTQGAQAAA GGAVDIYDMR EITSRMDFGQ QKIIVMALLC GCDYCPDGIG GIGKDGVLKL 240
FNKYKETEIL DRMRSWRGET DKYNALEIRV DDKSICSNCG HIGKTQSHTK SGCSVCRTHK 300
GCDESLWKEQ RLSIKSELTL RRKALLSPDF PNEEIIAEFL SEPDTIPNLN LNWRQPNLVK 360
FIKQIGHLLQ WPEIYCFQKF FPILTRWQVQ QSKQEKILIQ PHEIIKKRTV KGVPSLELRW 420
HDPSGIFKGL IPDKQIAEYE AEHPKGIEEL YYTIEPLDML ETAYPDLVAA FLKSKEKPAK 480
KTTRKKKTAS EEENKENEPN SKPKRVVRKI KAQPEENQPL LHQFLGRKKE GTPVKAPAPQ 540
RQQCSTPITK FLPSDLESDC DAEEFDMSDI VKGIISNPNA KPALTNHDGH QLHYEPMAED 600
LSLRLAQMSL GNVNESPKVE TKRDLSQVDQ LPQSKRFSLE DSFDLLVKGD LQKLARTPVE 660
RFKMQHRISE KIPTPVKPLD NISYFFNQSS DNADVFEELM NSSLVPQDQE DNAEDEEEDD 720
LVVISD 726 |
Gene Ontology | GO:0005634; C:nucleus; IDA:UniProtKB. GO:0035312; F:5'-3' exodeoxyribonuclease activity; IDA:FlyBase. GO:0003677; F:DNA binding; IEA:InterPro. GO:0008311; F:double-stranded DNA specific 3'-5' exodeoxyribonuclease activity; IDA:FlyBase. GO:0004520; F:endodeoxyribonuclease activity; IDA:FlyBase. GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. GO:0008310; F:single-stranded DNA specific 3'-5' exodeoxyribonuclease activity; IDA:FlyBase. GO:0000737; P:DNA catabolic process, endonucleolytic; IDA:FlyBase. GO:0000738; P:DNA catabolic process, exonucleolytic; IDA:FlyBase. GO:0006302; P:double-strand break repair; IMP:FlyBase. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |