CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017674
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 PDZ domain-containing protein 8 
Protein Synonyms/Alias
 Sarcoma antigen NY-SAR-84/NY-SAR-104 
Gene Name
 PDZD8 
Gene Synonyms/Alias
 PDZK8 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
49GEGFRYIKPVPGLLLubiquitination[1, 2, 3, 4, 5]
158GETVPFIKTIRLVRPubiquitination[1, 3, 4, 5, 6]
432TSTLQVLKLIKQAGDubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983
Functional Description
 Plays a role in the regulation of cell morphology and cytoskeletal organization. May inhibit herpes simplex virus 1 infection at an early stage. May promote retroviral infection. 
Sequence Annotation
 DOMAIN 366 449 PDZ.
 ZN_FING 840 891 Phorbol-ester/DAG-type.
 MOD_RES 496 496 Phosphoserine.
 MOD_RES 538 538 Phosphoserine.  
Keyword
 Coiled coil; Complete proteome; Host-virus interaction; Metal-binding; Phosphoprotein; Polymorphism; Reference proteome; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1154 AA 
Protein Sequence
MGLLLMILAS AVLGSFLTLL AQFFLLYRRQ PEPPADEAAR AGEGFRYIKP VPGLLLREYL 60
YGGGRDEEPS GAAPEGGATP TAAPETPAPP TRETCYFLNA TILFLFRELR DTALTRRWVT 120
KKIKVEFEEL LQTKTAGRLL EGLSLRDVFL GETVPFIKTI RLVRPVVPSA TGEPDGPEGE 180
ALPAACPEEL AFEAEVEYNG GFHLAIDVDL VFGKSAYLFV KLSRVVGRLR LVFTRVPFTH 240
WFFSFVEDPL IDFEVRSQFE GRPMPQLTSI IVNQLKKIIK RKHTLPNYKI RFKPFFPYQT 300
LQGFEEDEEH IHIQQWALTE GRLKVTLLEC SRLLIFGSYD REANVHCTLE LSSSVWEEKQ 360
RSSIKTVELI KGNLQSVGLT LRLVQSTDGY AGHVIIETVA PNSPAAIADL QRGDRLIAIG 420
GVKITSTLQV LKLIKQAGDR VLVYYERPVG QSNQGAVLQD NFGQLEENFL SSSCQSGYEE 480
EAAGLTVDTE SRELDSEFED LASDVRAQNE FKDEAQSLSH SPKRVPTTLS IKPLGAISPV 540
LNRKLAVGSH PLPPKIQSKD GNKPPPLKTS EITDPAQVSK PTQGSAFKPP VPPRPQAKVP 600
LPSADAPNQA EPDVLVEKPE KVVPPPLVDK SAEKQAKNVD AIDDAAAPKQ FLAKQEVAKD 660
VTSETSCPTK DSSDDRQTWE SSEILYRNKL GKWTRTRASC LFDIEACHRY LNIALWCRDP 720
FKLGGLICLG HVSLKLEDVA LGCLATSNTE YLSKLRLEAP SPKAIVTRTA LRNLSMQKGF 780
NDKFCYGDIT IHFKYLKEGE SDHHVVTNVE KEKEPHLVEE VSVLPKEEQF VGQMGLTENK 840
HSFQDTQFQN PTWCDYCKKK VWTKAASQCM FCAYVCHKKC QEKCLAETSV CGATDRRIDR 900
TLKNLRLEGQ ETLLGLPPRV DAEASKSVNK TTGLTRHIIN TSSRLLNLRQ VSKTRLSEPG 960
TDLVEPSPKH TPNTSDNEGS DTEVCGPNSP SKRGNSTGIK LVRKEGGLDD SVFIAVKEIG 1020
RDLYRGLPTE ERIQKLEFML DKLQNEIDQE LEHNNSLVRE EKETTDTRKK SLLSAALAKS 1080
GERLQALTLL MIHYRAGIED IETLESLSLD QHSKKISKYT DDTEEDLDNE ISQLIDSQPF 1140
SSISDDLFGP SESV 1154 
Gene Ontology
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0007010; P:cytoskeleton organization; IMP:UniProtKB.
 GO:0035556; P:intracellular signal transduction; IEA:InterPro.
 GO:0022604; P:regulation of cell morphogenesis; IMP:UniProtKB.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 
Interpro
 IPR001478; PDZ.
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd. 
Pfam
 PF00130; C1_1
 PF13180; PDZ_2 
SMART
 SM00109; C1
 SM00228; PDZ 
PROSITE
 PS50106; PDZ
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 
PRINTS