UniProt Accession

 RPC3_HUMAN; Q9BUI4; O15317; Q9Y3R6 

Genbank Protein ID

 U93867; AY091463; AJ238221; AJ238222; AJ238223; AJ238224; AJ238225; AJ238226; AJ238227; AJ238228; AJ238229; AJ238230; AJ238231; AJ238232; AJ238233; AJ238234; BC002586 

Genbank Nucleotide ID

 AAB63675.1; AAM12033.1; CAB41919.1; CAB41919.1; CAB41919.1; CAB41919.1; CAB41919.1; CAB41919.1; CAB41919.1; CAB41919.1; CAB41919.1; CAB41919.1; CAB41919.1; CAB41919.1; CAB41919.1; CAB41919.1; AAH02586.1 

Protein Name

 DNA-directed RNA polymerase III subunit RPC3 

Protein Synonyms/Alias

 RNA polymerase III subunit C3; DNA-directed RNA polymerase III subunit C; RNA polymerase III 62 kDa subunit; RPC62 

Gene Name

 POLR3C 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
51	GTSLDQVKKALCVLV	ubiquitination	[1]
192	KDMYLVPKLSLIGKG	ubiquitination	[1, 2, 3]
225	PKYTTDNKEPIPDDG	ubiquitination	[1, 4]
378	QKKHIEQKQVEDFAM	ubiquitination	[1]
389	DFAMIPAKEAKDMLY	ubiquitination	[1]
392	MIPAKEAKDMLYKML	ubiquitination	[1]
411	MSLQEIPKTPDHAPS	ubiquitination	[1]
441	MLLHRCYKSIANLIE	ubiquitination	[1]
503	RQQLETLKRNVNKLD	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB- DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. 

Sequence Annotation

 MOD_RES 194 194 Phosphoserine.  

Keyword

 3D-structure; Antiviral defense; Complete proteome; DNA-directed RNA polymerase; Immunity; Innate immunity; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 534 AA 

Protein Sequence

Gene Ontology

 GO:0005666; C:DNA-directed RNA polymerase III complex; TAS:ProtInc.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003899; F:DNA-directed RNA polymerase activity; TAS:ProtInc.
 GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
 GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
 GO:0045089; P:positive regulation of innate immune response; IMP:UniProtKB.
 GO:0032728; P:positive regulation of interferon-beta production; IMP:UniProtKB.
 GO:0006359; P:regulation of transcription from RNA polymerase III promoter; TAS:ProtInc.
 GO:0006386; P:termination of RNA polymerase III transcription; TAS:Reactome.
 GO:0006385; P:transcription elongation from RNA polymerase III promoter; TAS:Reactome. 

Interpro

 IPR013197; RNA_pol_III_RPC82-rel_HTH.
 IPR008806; RNA_pol_III_Rpc82_C. 

Pfam

 PF08221; HTH_9
 PF05645; RNA_pol_Rpc82 

SMART

  

PROSITE

  

PRINTS