CPLM-020959

Genbank Nucleotide ID

Protein Synonyms/Alias

Deoxycytidylate kinase; CK; dCMP kinase; Uridine monophosphate/cytidine monophosphate kinase; UMP/CMP kinase; UMP/CMPK

Cmk; Cmpk; Uck; Umk; Umpk

Mus musculus (Mouse)

Position	Peptide	Type	References
2	******MKPLVVFVL	ubiquitination	[1]
26	QCARIVEKYGYTHLS	ubiquitination	[1]
43	ELLRDERKNPDSQYG	acetylation	[2, 3, 4, 5]
43	ELLRDERKNPDSQYG	ubiquitination	[1]
55	QYGELIEKYIKEGKI	acetylation	[6]
55	QYGELIEKYIKEGKI	ubiquitination	[1]
88	AANAQKNKFLIDGFP	ubiquitination	[1]
106	DNLQGWNKTMDGKAD	acetylation	[4, 5]
106	DNLQGWNKTMDGKAD	succinylation	[5]
106	DNLQGWNKTMDGKAD	ubiquitination	[1]
190	EVFGEVVKIFDKEG*	ubiquitination	[1]

[1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
[2] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
[3] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
Simon GM, Cheng J, Gordon JI.
Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
[4] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
[5] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
[6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]

Functional Description

Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. Plays an important role in de novo pyrimidine nucleotide biosynthesis. Has preference for UMP and CMP as phosphate acceptors (By similarity).

NP_BIND 13 18 ATP (By similarity).
NP_BIND 61 63 NMP (By similarity).
NP_BIND 93 96 NMP (By similarity).
REGION 33 63 NMPbind (By similarity).
REGION 133 143 LID (By similarity).
BINDING 39 39 NMP (By similarity).
BINDING 100 100 CMP (By similarity).
BINDING 134 134 ATP (By similarity).
BINDING 140 140 NMP (By similarity).
BINDING 151 151 NMP (By similarity).
BINDING 179 179 ATP; via carbonyl oxygen (By similarity).
MOD_RES 55 55 N6-acetyllysine (By similarity).

Acetylation; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Nucleotide-binding; Nucleus; Pyrimidine biosynthesis; Reference proteome; Transferase.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO:0004127; F:cytidylate kinase activity; IEA:EC.
GO:0033862; F:UMP kinase activity; IEA:Compara.
GO:0046705; P:CDP biosynthetic process; IEA:Compara.
GO:0006240; P:dCDP biosynthetic process; IEA:Compara.
GO:0006227; P:dUDP biosynthetic process; IEA:Compara.
GO:0022602; P:ovulation cycle process; IEA:Compara.
GO:0018963; P:phthalate metabolic process; IEA:Compara.
GO:0006225; P:UDP biosynthetic process; IEA:Compara.

IPR000850; Adenylate_kin.
IPR027417; P-loop_NTPase.
IPR006266; UMP_CMP_kinase.

PS00113; ADENYLATE_KINASE

PR00094; ADENYLTKNASE.