UniProt Accession

 CMOA_ECOLI; P76290; O07982 

Genbank Protein ID

 U00096; AP009048 

Genbank Nucleotide ID

 AAC74940.1; BAA15680.1 

Protein Name

 tRNA (cmo5U34)-methyltransferase 

Protein Synonyms/Alias

  

Gene Name

 cmoA 

Gene Synonyms/Alias

 yecO; b1870; JW1859 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
172	KFSFEDAKVGELLFN	acetylation	[1]
185	FNMHHDFKRANGYSE	acetylation	[1]
198	SELEISQKRSMLENV	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes the conversion of 5-methoxyuridine (mo5U) to uridine-5-oxyacetic acid (cmo5U) at position 34 in tRNA. May also participate in the methylation of uridine-5-oxyacetic acid (cmo5U) to uridine-5-oxyacetic acid methyl ester (mcmo5U) (By similarity). 

Sequence Annotation

 REGION 64 66 S-adenosyl-L-methionine binding (By
 REGION 117 118 S-adenosyl-L-methionine binding (By
 BINDING 39 39 S-adenosyl-L-methionine (By similarity).
 BINDING 89 89 S-adenosyl-L-methionine (By similarity).
 BINDING 132 132 S-adenosyl-L-methionine (By similarity).
 BINDING 199 199 Substrate (Potential).  

Keyword

 3D-structure; Complete proteome; Methyltransferase; Reference proteome; S-adenosyl-L-methionine; Transferase; tRNA processing. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 247 AA 

Protein Sequence

Gene Ontology

 GO:0008757; F:S-adenosylmethionine-dependent methyltransferase activity; IEA:HAMAP.
 GO:0016300; F:tRNA (uracil) methyltransferase activity; IEA:InterPro.
 GO:0002098; P:tRNA wobble uridine modification; IEA:InterPro. 

Interpro

 IPR005271; tRNA_cmo5U34_MeTrfase. 

Pfam

  

SMART

  

PROSITE

  

PRINTS