UniProt Accession

 F262_HUMAN; O60825; O60824; Q5VVQ3; Q5VVQ4; Q9H3P1 

Genbank Protein ID

 AJ005577; AJ005578; AB044805; AF470623; AK292883; AL445493; AL445493; CH471100; BC069350; BC069385; BC069583; BC069586; BC069600; BC075075; BC075076; BC112103; BC112105 

Genbank Nucleotide ID

 CAA06605.1; CAA06606.1; BAB19681.1; AAL99386.1; BAF85572.1; CAH70777.1; CAH70778.1; EAW93508.1; AAH69350.1; AAH69385.1; AAH69583.1; AAH69586.1; AAH69600.1; AAH75075.1; AAH75076.1; AAI12104.1; AAI12106.1 

Protein Name

 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 

Protein Synonyms/Alias

 6PF-2-K/Fru-2,6-P2ase 2; PFK/FBPase 2; 6PF-2-K/Fru-2,6-P2ase heart-type isozyme; 6-phosphofructo-2-kinase; Fructose-2,6-bisphosphatase 

Gene Name

 PFKFB2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
305	KVWTSQLKRTIQTAE	ubiquitination	[1, 2]

Reference

 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Synthesis and degradation of fructose 2,6-bisphosphate. 

Sequence Annotation

 NP_BIND 45 52 ATP (Potential).
 REGION 2 248 6-phosphofructo-2-kinase.
 REGION 249 505 Fructose-2,6-bisphosphatase.
 ACT_SITE 128 128 Potential.
 ACT_SITE 158 158 Potential.
 ACT_SITE 257 257 Tele-phosphohistidine intermediate (By
 ACT_SITE 326 326 Potential.
 ACT_SITE 391 391 Proton donor (By similarity).
 BINDING 102 102 Fructose-6-phosphate (By similarity).
 BINDING 193 193 Fructose-6-phosphate (By similarity).
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 29 29 Phosphoserine; by PKA (By similarity).
 MOD_RES 466 466 Phosphoserine; by AMPK.
 MOD_RES 468 468 Phosphothreonine.
 MOD_RES 475 475 Phosphothreonine; by PKC (By similarity).
 MOD_RES 483 483 Phosphoserine.
 MOD_RES 493 493 Phosphoserine.  

Keyword

 Acetylation; Alternative splicing; ATP-binding; Complete proteome; Hydrolase; Kinase; Multifunctional enzyme; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 505 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0003873; F:6-phosphofructo-2-kinase activity; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004331; F:fructose-2,6-bisphosphate 2-phosphatase activity; IEA:EC.
 GO:0006003; P:fructose 2,6-bisphosphate metabolic process; IEA:InterPro.
 GO:0006000; P:fructose metabolic process; TAS:ProtInc.
 GO:0006096; P:glycolysis; IDA:UniProtKB.
 GO:0006089; P:lactate metabolic process; IEA:Compara.
 GO:0033133; P:positive regulation of glucokinase activity; IEA:Compara.
 GO:0032024; P:positive regulation of insulin secretion; IEA:Compara.
 GO:0006090; P:pyruvate metabolic process; IEA:Compara.
 GO:0009749; P:response to glucose stimulus; IEA:Compara.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 

Interpro

 IPR003094; 6Pfruct_kin.
 IPR013079; 6Phosfructo_kin.
 IPR016260; Bifunct_6PFK/fruc_bisP_Ptase.
 IPR013078; His_Pase_superF_clade-1.
 IPR027417; P-loop_NTPase.
 IPR001345; PG/BPGM_mutase_AS. 

Pfam

 PF01591; 6PF2K
 PF00300; His_Phos_1 

SMART

 SM00855; PGAM 

PROSITE

 PS00175; PG_MUTASE 

PRINTS

 PR00991; 6PFRUCTKNASE.