CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021130
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 AF4/FMR2 family member 4 
Protein Synonyms/Alias
  
Gene Name
 Aff4 
Gene Synonyms/Alias
 Alf4 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
63LGNYDEMKDYIGDRSubiquitination[1]
247KSSSMLQKPTAYVRPubiquitination[1]
283GNSMTELKPSSKAHLubiquitination[1]
339PCKTEPSKFPFPTKEubiquitination[1]
345SKFPFPTKESQQSNFubiquitination[1]
578EEPRGGLKIESETPVubiquitination[1]
817PAGPILSKDSKTEHGacetylation[2, 3]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
 Key component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through direct interactions with ELL proteins (ELL, ELL2 or ELL3) and the P-TEFb complex. In case of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression (By similarity). 
Sequence Annotation
 MOD_RES 382 382 Phosphoserine (By similarity).
 MOD_RES 383 383 Phosphoserine (By similarity).
 MOD_RES 384 384 Phosphoserine (By similarity).
 MOD_RES 387 387 Phosphoserine (By similarity).
 MOD_RES 544 544 Phosphoserine (By similarity).
 MOD_RES 666 666 Phosphoserine (By similarity).
 MOD_RES 669 669 Phosphothreonine (By similarity).
 MOD_RES 689 689 Phosphoserine (By similarity).
 MOD_RES 698 698 Phosphoserine (By similarity).
 MOD_RES 701 701 Phosphoserine (By similarity).
 MOD_RES 707 707 Phosphotyrosine (By similarity).
 MOD_RES 809 809 Phosphoserine (By similarity).
 MOD_RES 831 831 Phosphoserine (By similarity).
 MOD_RES 1040 1040 Phosphoserine (By similarity).
 MOD_RES 1052 1052 Phosphoserine (By similarity).
 MOD_RES 1055 1055 Phosphoserine (By similarity).
 MOD_RES 1059 1059 Phosphoserine (By similarity).  
Keyword
 Complete proteome; Nucleus; Phosphoprotein; Proto-oncogene; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1160 AA 
Protein Sequence
MNREDRNVLR MKERERRNQE IQQGEDAFPP SSPLFAEPYK VTSKEDKLSS RIQSMLGNYD 60
EMKDYIGDRS IPKLVAIPKP AVPTTTDEKA NPNFFEQRHG GSHQSSKWTP VGPAPSTSQS 120
QKRSSALQSG HSSQRSGAGG SGASSSGQRH DRDSYSSSRK KGQHGSEHSK SRSSSPGKPQ 180
AVSSLSSSHS RSHGNDHHSK EHQRSKSPRD PDANWDSPSR GPFSSGQHSS QSFPPSLMSK 240
SSSMLQKPTA YVRPMDGQES VEPKLSSEHY SSQSHGNSMT ELKPSSKAHL TKLKIPSRPL 300
DASVSGDVSC VDEILKEMTH SWPPPLTAIH TPCKTEPSKF PFPTKESQQS NFGPGEQKRY 360
STAKTSNGHQ SKSMLKDDLK LSSSEDSDGE QDCDKTMPRS TPGSNSEPSH HNSEGADNSR 420
DDSSSHSGSE SSSGSDSESE SSSSDSEANE PSQSASPEPE PPPTNKWQLD NWLNKVNPHK 480
VSPASSVDSN IPSSQAYKKE GREQGTASNY TDPGGTKETS SATPGRDSKT IQKGSESGRG 540
RQKSPAQSDS TTQRRTVGKK QPKKPEKSAA EEPRGGLKIE SETPVDMAAS MPSSRHKAAT 600
KGSRKPNIKK ESKSSPRPTA EKKKYKSASK PSQKSREIIE TDTSSSDSDG SESLPPSSQT 660
PKYPESNRTP VKPSSVEEED SFFRQRMFSP MEEKELLSPL SEPDDRYPLI VKIDLNLLTR 720
IPGKPYKETE PPKGEKKNVP EKHSREVQKQ ASEKASNKGK RKHKNDDDTR ASESKKPKTE 780
DKNSSGHKPS SSRESSKQSS TKEKDLLPSP AGPILSKDSK TEHGSRKRTV SQSSSLKSSG 840
TSSKENSGSS SKSSSSSTAK QKKTEGKGPS SSKEAKEKAP NSSSNCPPST PTSESSKPRR 900
TKLAFDDRNY SADHYLQEAK KLKHNADALS DRFEKAVYYL DAVVSFIECG NALEKNAQES 960
KSPFPMYSDT VELIKYTMKL KNYLAPDATA ADKRLTVLCL RCQSLLYLRL FKLKKENALK 1020
YSKTLTEHLK NSYSNSQAPS PGLGSKAVGM PSPVSPKLSP GNSGSYSSGG SSASASGSSV 1080
TIPQKIHQMA ASYVQVTSNF LYATEIWDQA EQLSKEQKEF FAELDKVMGP LIFNASIMTD 1140
LARYTRQGLH WLRQDAKLIS 1160 
Gene Ontology
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0007286; P:spermatid development; IMP:MGI.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR007797; TF_AF4/FMR2. 
Pfam
 PF05110; AF-4 
SMART
  
PROSITE
  
PRINTS