UniProt Accession

 PSA3_YEAST; P23638; D6VUR7 

Genbank Protein ID

 M63851; X56730; Z72920; BK006941 

Genbank Nucleotide ID

 AAA34907.1; CAA40054.1; CAA97148.1; DAA08228.1 

Protein Name

 Proteasome subunit alpha type-3 

Protein Synonyms/Alias

 Macropain subunit Y13; Multicatalytic endopeptidase complex subunit Y13; Proteasome component Y13; Proteinase YSCE subunit 13 

Gene Name

 PRE9 

Gene Synonyms/Alias

 PRS5; YGR135W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
51	IVLAAERKVTSTLLE	ubiquitination	[1]
65	EQDTSTEKLYKLNDK	ubiquitination	[1]
100	IHAQNYLKTYNEDIP	ubiquitination	[1, 2]
199	LALKTLSKTTDSSAL	ubiquitination	[1, 2, 3, 4, 5, 6]
231	EVYQKIFKPQEIKDI	ubiquitination	[1, 2]
236	IFKPQEIKDILVKTG	ubiquitination	[1, 2]
246	LVKTGITKKDEDEEA	ubiquitination	[1]
247	VKTGITKKDEDEEAD	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery.
 Hitchcock AL, Auld K, Gygi SP, Silver PA.
 Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12735-40. [PMID: 14557538]
 [4] A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking.
 Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
 Mol Cell Proteomics. 2006 Apr;5(4):737-48. [PMID: 16432255]
 [5] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [6] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 

Functional Description

 The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity. 

Sequence Annotation

 CROSSLNK 199 199 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Isopeptide bond; Nucleus; Protease; Proteasome; Reference proteome; Threonine protease; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 258 AA 

Protein Sequence

Gene Ontology

 GO:0042175; C:nuclear outer membrane-endoplasmic reticulum membrane network; IC:SGD.
 GO:0005634; C:nucleus; IC:SGD.
 GO:0019773; C:proteasome core complex, alpha-subunit complex; IDA:SGD.
 GO:0034515; C:proteasome storage granule; IDA:SGD.
 GO:0004298; F:threonine-type endopeptidase activity; IEA:UniProtKB-KW.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IDA:SGD.
 GO:0010499; P:proteasomal ubiquitin-independent protein catabolic process; IDA:SGD.
 GO:0080129; P:proteasome core complex assembly; IMP:SGD. 

Interpro

 IPR000426; Proteasome_asu_N.
 IPR016050; Proteasome_bsu_CS.
 IPR023332; Proteasome_suA-type.
 IPR001353; Proteasome_sua/b. 

Pfam

 PF00227; Proteasome
 PF10584; Proteasome_A_N 

SMART

 SM00948; Proteasome_A_N 

PROSITE

 PS00388; PROTEASOME_A_1
 PS51475; PROTEASOME_A_2 

PRINTS