UniProt Accession

 FNBP1_MOUSE; Q80TY0; A2AQ40; A2AQ46; Q3TA45; Q3TCW9; Q3U081; Q3UPI7; Q8C727; Q99L37 

Genbank Protein ID

 AK122308; AK052652; AK143512; AK157135; AK170497; AK172100; AL844546; AL844546; BC003867 

Genbank Nucleotide ID

 BAC65590.1; BAC35082.1; BAE25408.1; BAE33974.1; BAE41836.1; BAE42825.1; CAM18436.1; CAM18442.1; AAH03867.1 

Protein Name

 Formin-binding protein 1 

Protein Synonyms/Alias

 Formin-binding protein 17 

Gene Name

 Fnbp1 

Gene Synonyms/Alias

 Fbp17; Kiaa0554 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
166	DADINVTKADVEKAR	ubiquitination	[1]
319	FGGKSRGKLWPFIKK	ubiquitination	[1]
377	FNEFMTSKPKIHCFR	ubiquitination	[1]
456	DPASLDQKLTEVTQN	ubiquitination	[1]
611	YVEVYLDKNAKGS**	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during the late stage of clathrin-mediated endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also enhances actin polymerization via the recruitment of WASL/N-WASP, which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. May act as a link between RND2 signaling and regulation of the actin cytoskeleton. May be required for the lysosomal retention of FASLG/FASL (By similarity). 

Sequence Annotation

 DOMAIN 1 65 FCH.
 REPEAT 415 490 REM.
 DOMAIN 549 610 SH3.
 REGION 1 334 Interaction with microtubules (By
 REGION 1 288 F-BAR domain (By similarity).
 REGION 1 79 Required for self-association and
 REGION 251 616 Required for self-association and
 REGION 399 551 Interaction with RND2 (By similarity).
 REGION 494 616 Interaction with PDE6G (By similarity).
 REGION 513 616 Required for interaction with TNKS (By
 REGION 534 616 Interaction with DNM1 and DNM3 (By
 REGION 549 616 Interaction with ARHGAP17, DAAM1, DIAPH1
 REGION 552 609 Interaction with FASLG (By similarity).
 REGION 552 608 Interaction with DNM2 and WASL (By
 MOD_RES 66 66 N6-acetyllysine (By similarity).
 MOD_RES 110 110 N6-acetyllysine (By similarity).
 MOD_RES 294 294 Phosphothreonine.
 MOD_RES 296 296 Phosphoserine.
 MOD_RES 299 299 Phosphoserine.
 MOD_RES 348 348 Phosphoserine (By similarity).
 MOD_RES 358 358 Phosphoserine (By similarity).
 MOD_RES 496 496 Phosphoserine.
 MOD_RES 499 499 Phosphotyrosine.  

Keyword

 Acetylation; Alternative splicing; Cell membrane; Coated pit; Coiled coil; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Cytoskeleton; Endocytosis; Lipid-binding; Lysosome; Membrane; Phosphoprotein; Reference proteome; SH3 domain. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 616 AA 

Protein Sequence

Gene Ontology

 GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
 GO:0005905; C:coated pit; IEA:UniProtKB-SubCell.
 GO:0016023; C:cytoplasmic membrane-bounded vesicle; IEA:UniProtKB-SubCell.
 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0005764; C:lysosome; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
 GO:0006897; P:endocytosis; IEA:UniProtKB-KW. 

Interpro

 IPR001060; FCH_dom.
 IPR001452; SH3_domain. 

Pfam

 PF00611; FCH
 PF00018; SH3_1 

SMART

 SM00055; FCH
 SM00326; SH3 

PROSITE

 PS50133; FCH
 PS50002; SH3 

PRINTS