UniProt Accession

 MET17_YEAST; P06106; D6VYU6 

Genbank Protein ID

 X04493; U17243; AY723848; BK006945 

Genbank Nucleotide ID

 CAA28181.1; AAB67347.1; AAU09765.1; DAA09612.1 

Protein Name

 Protein MET17 

Protein Synonyms/Alias

 O-acetylhomoserine sulfhydrylase; OAH sulfhydrylase; Homocysteine synthase; O-acetylserine sulfhydrylase; OAS sulfhydrylase 

Gene Name

 MET17 

Gene Synonyms/Alias

 MET25; YLR303W; L8003.1 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
142	DNPEEFEKVFDERTK	acetylation	[1]
160	LETIGNPKYNVPDFE	acetylation	[1]
160	LETIGNPKYNVPDFE	ubiquitination	[2, 3]
168	YNVPDFEKIVAIAHK	acetylation	[1]
236	PWKDYPEKFPQFSQP	ubiquitination	[4]
305	RHGENALKLAKWLEQ	acetylation	[1]
357	KDLPNADKETDPFKL	acetylation	[1]
363	DKETDPFKLSGAQVV	acetylation	[1]
398	APYFTTHKQLNDKEK	acetylation	[1]
412	KLASGVTKDLIRVSV	acetylation	[1]
412	KLASGVTKDLIRVSV	ubiquitination	[3]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [4] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 

Functional Description

 Transforms O-acetylhomoserine into homocysteine and O- acetylserine into cysteine. 

Sequence Annotation

 MOD_RES 44 44 Phosphoserine.
 MOD_RES 209 209 N6-(pyridoxal phosphate)lysine (By  

Keyword

 Amino-acid biosynthesis; Complete proteome; Cysteine biosynthesis; Cytoplasm; Direct protein sequencing; Methionine biosynthesis; Multifunctional enzyme; Phosphoprotein; Pyridoxal phosphate; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 444 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0004124; F:cysteine synthase activity; IDA:SGD.
 GO:0003961; F:O-acetylhomoserine aminocarboxypropyltransferase activity; IDA:SGD.
 GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro. 

Interpro

 IPR000277; Cys/Met-Metab_PyrdxlP-dep_enz.
 IPR006235; OAc-hSer/O-AcSer_sulfhydrylase.
 IPR015424; PyrdxlP-dep_Trfase.
 IPR015421; PyrdxlP-dep_Trfase_major_sub1.
 IPR015422; PyrdxlP-dep_Trfase_major_sub2. 

Pfam

 PF01053; Cys_Met_Meta_PP 

SMART

  

PROSITE

 PS00868; CYS_MET_METAB_PP 

PRINTS