UniProt Accession

 CRYAA_BOVIN; P02470 

Genbank Protein ID

 M26142 

Genbank Nucleotide ID

 AAA30471.1 

Protein Name

 Alpha-crystallin A chain 

Protein Synonyms/Alias

 Alpha-crystallin A chain, short form 

Gene Name

 CRYAA 

Gene Synonyms/Alias

 CRYA1 

Created Date

 July 27, 2013 

Organism

 Bos taurus (Bovine) 

NCBI Taxa ID

 9913 

Lysine Modification

Position	Peptide	Type	References
11	AIQHPWFKRTLGPFY	glycation	[1]
78	FVIFLDVKHFSPEDL	glycation	[1]
88	SPEDLTVKVQEDFVE	glycation	[2, 3]
99	DFVEIHGKHNERQDD	glycation	[2, 3]
166	IPVSREEKPSSAPSS	glycation	[4]

Reference

 [1] Site selectivity in the glycation of alpha A- and alpha B-crystallins by glucose.
 Abraham EC, Cherian M, Smith JB.
 Biochem Biophys Res Commun. 1994 Jun 30;201(3):1451-6. [PMID: 7912928]
 [2] Quantification of post-translationally modified peptides of bovine alpha-crystallin using tandem mass tags and electron transfer dissociation.
 Viner RI, Zhang T, Second T, Zabrouskov V.
 J Proteomics. 2009 Jul 21;72(5):874-85. [PMID: 19245863]
 [3] Mapping of O-linked beta-N-acetylglucosamine modification sites in key contractile proteins of rat skeletal muscle.
 Hédou J, Bastide B, Page A, Michalski JC, Morelle W.
 Proteomics. 2009 Apr;9(8):2139-48. [PMID: 19322778]
 [4] Role of the specifically targeted lysine residues in the glycation dependent loss of chaperone activity of alpha A- and alpha B-crystallins.
 Abraham EC, Huaqian J, Aziz A, Kumarasamy A, Datta P.
 Mol Cell Biochem. 2008 Mar;310(1-2):235-9. [PMID: 18158587] 

Functional Description

 May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. 

Sequence Annotation

 METAL 79 79 Zinc (By similarity).
 METAL 107 107 Zinc (By similarity).
 METAL 115 115 Zinc (By similarity).
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 21 21 Omega-N-methylated arginine (By
 MOD_RES 70 70 N6-acetyllysine (By similarity).
 MOD_RES 99 99 N6-acetyllysine (By similarity).
 MOD_RES 122 122 Phosphoserine.
 CARBOHYD 11 11 N-linked (Glc) (glycation).
 CARBOHYD 78 78 N-linked (Glc) (glycation).
 CARBOHYD 162 162 O-linked (GlcNAc).  

Keyword

 3D-structure; Acetylation; Chaperone; Complete proteome; Cytoplasm; Direct protein sequencing; Eye lens protein; Glycation; Glycoprotein; Metal-binding; Methylation; Nucleus; Oxidation; Phosphoprotein; Reference proteome; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 173 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0005212; F:structural constituent of eye lens; IEA:UniProtKB-KW.
 GO:0051082; F:unfolded protein binding; IDA:UniProtKB.
 GO:0007015; P:actin filament organization; IEA:Compara.
 GO:0060561; P:apoptotic process involved in morphogenesis; IEA:Compara.
 GO:0048596; P:embryonic camera-type eye morphogenesis; IEA:Compara.
 GO:0070309; P:lens fiber cell morphogenesis; IEA:Compara.
 GO:0000072; P:M phase specific microtubule process; IEA:Compara.
 GO:0007005; P:mitochondrion organization; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
 GO:0043154; P:negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; IEA:Compara.
 GO:0010629; P:negative regulation of gene expression; IEA:Compara.
 GO:0032387; P:negative regulation of intracellular transport; IEA:Compara.
 GO:0030307; P:positive regulation of cell growth; IEA:Compara.
 GO:0001934; P:positive regulation of protein phosphorylation; IEA:Compara.
 GO:0051260; P:protein homooligomerization; IEA:Compara.
 GO:0042542; P:response to hydrogen peroxide; IEA:Compara.
 GO:0001666; P:response to hypoxia; IEA:Compara.
 GO:0070141; P:response to UV-A; IEA:Compara.
 GO:0007021; P:tubulin complex assembly; IEA:Compara.
 GO:0007601; P:visual perception; IEA:Compara. 

Interpro

 IPR002068; a-crystallin/Hsp20_dom.
 IPR001436; Alpha-crystallin/HSP.
 IPR012274; Alpha-crystallin_A.
 IPR003090; Alpha-crystallin_N.
 IPR008978; HSP20-like_chaperone. 

Pfam

 PF00525; Crystallin
 PF00011; HSP20 

SMART

  

PROSITE

 PS01031; HSP20 

PRINTS

 PR00299; ACRYSTALLIN.