| Tag | Content |
|---|
| CPLM ID | CPLM-003527 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Nitrate/nitrite sensor protein NarX |
Protein Synonyms/Alias | |
Gene Name | narX |
Gene Synonyms/Alias | narR; b1222; JW1213 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 331 | GDRGTTLKWRLADSH | acetylation | [1] |
|
Reference | [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] |
Functional Description | Acts as a sensor for nitrate/nitrite and transduces signal of nitrate availability to the NarL protein and of both nitrate/nitrite to the NarP protein. NarX probably activates NarL and NarP by phosphorylation in the presence of nitrate. NarX also plays a negative role in controlling NarL activity, probably through dephosphorylation in the absence of nitrate. |
Sequence Annotation | DOMAIN 176 228 HAMP.
DOMAIN 393 587 Histidine kinase.
MOD_RES 399 399 Phosphohistidine; by autocatalysis (By |
Keyword | 3D-structure; ATP-binding; Cell inner membrane; Cell membrane; Complete proteome; Kinase; Membrane; Nitrate assimilation; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase; Transmembrane; Transmembrane helix; Two-component regulatory system. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 598 AA |
Protein Sequence | MLKRCLSPLT LVNQVALIVL LSTAIGLAGM AVSGWLVQGV QGSAHAINKA GSLRMQSYRL 60
LAAVPLSEKD KPLIKEMEQT AFSAELTRAA ERDGQLAQLQ GLQDYWRNEL IPALMRAQNR 120
ETVSADVSQF VAGLDQLVSG FDRTTEMRIE TVVLVHRVMA VFMALLLVFT IIWLRARLLQ 180
PWRQLLAMAS AVSHRDFTQR ANISGRNEMA MLGTALNNMS AELAESYAVL EQRVQEKTAG 240
LEHKNQILSF LWQANRRLHS RAPLCERLSP VLNGLQNLTL LRDIELRVYD TDDEENHQEF 300
TCQPDMTCDD KGCQLCPRGV LPVGDRGTTL KWRLADSHTQ YGILLATLPQ GRHLSHDQQQ 360
LVDTLVEQLT ATLALDRHQE RQQQLIVMEE RATIARELHD SIAQSLSCMK MQVSCLQMQG 420
DALPESSREL LSQIRNELNA SWAQLRELLT TFRLQLTEPG LRPALEASCE EYSAKFGFPV 480
KLDYQLPPRL VPSHQAIHLL QIAREALSNA LKHSQASEVV VTVAQNDNQV KLTVQDNGCG 540
VPENAIRSNH YGMIIMRDRA QSLRGDCRVR RRESGGTEVV VTFIPEKTFT DVQGDTHE 598 |
Gene Ontology | GO:0016021; C:integral to membrane; IEA:UniProtKB-KW. GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell. GO:0005524; F:ATP binding; IEA:UniProtKB-KW. GO:0004721; F:phosphoprotein phosphatase activity; IDA:EcoCyc. GO:0000155; F:phosphorelay sensor kinase activity; IDA:EcoCyc. GO:0071249; P:cellular response to nitrate; IEP:EcoCyc. GO:0071250; P:cellular response to nitrite; IMP:EcoCyc. GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW. GO:0046777; P:protein autophosphorylation; IDA:EcoCyc. GO:0006974; P:response to DNA damage stimulus; IEP:EcoliWiki. |
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