| Tag | Content |
|---|
| CPLM ID | CPLM-012220 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Rap guanine nucleotide exchange factor 1 |
Protein Synonyms/Alias | CRK SH3-binding GNRP; Guanine nucleotide-releasing factor 2; Protein C3G |
Gene Name | RAPGEF1 |
Gene Synonyms/Alias | GRF2 |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 218 | KAVLDGVKELVRLTI | ubiquitination | [1] | | 701 | IMSRLTLKQEGDDGP | ubiquitination | [1] | | 753 | SPEELIKKLQYRYEK | ubiquitination | [1] | | 831 | LDKVDQKKLLRCATS | ubiquitination | [1] |
|
Reference | [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA. Mol Cell Proteomics. 2013 Mar;12(3):825-31. [ PMID: 23266961] |
Functional Description | Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. |
Sequence Annotation | DOMAIN 688 810 N-terminal Ras-GEF.
DOMAIN 840 1064 Ras-GEF.
MOTIF 281 292 SH3-binding.
MOTIF 451 462 SH3-binding.
MOTIF 538 549 SH3-binding.
MOTIF 606 617 SH3-binding.
MOD_RES 293 293 Phosphoserine.
MOD_RES 360 360 Phosphoserine.
MOD_RES 504 504 Phosphotyrosine; by HCK. |
Keyword | Alternative splicing; Complete proteome; Guanine-nucleotide releasing factor; Phosphoprotein; Polymorphism; Reference proteome; SH3-binding. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1077 AA |
Protein Sequence | MGNAIEKQKP LKRSHLYPWK QDSQRSHLSS FTMKLMDKFH SPKIKRTPSK KGKPAEVSVK 60
IPEKPVNKEA TDRFLPEGYP LPLDLEQQAV EFMSTSAVAS RSQRQKNLSW LEEKEKEVVS 120
ALRYFKTIVD KMAIDKKVLE MLPGSASKVL EAILPLVQND PRIQHSSALS SCYSRVYQSL 180
ANLIRWSDQV MLEGVNSEDK EMVTTVKGVI KAVLDGVKEL VRLTIEKQGR PSPTSPVKPS 240
SPASKPDGPA ELPLTDREVE ILNKTTGMSQ STELLPDATD EEVAPPKPPL PGIRVVDNSP 300
PPALPPKKRQ SAPSPTRVAV VAPMSRATSG SSLPVGINRQ DFDVDCYAQR RLSGGSHSYG 360
GESPRLSPCS SIGKLSKSDE QLSSLDRDSG QCSRNTSCET LDHYDPDYEF LQQDLSNADQ 420
IPQQTAWNLS PLPESLGESG SPFLGPPFQL PLGGHPQPDG PLAPGQQTDT PPALPEKKRR 480
SAASQTADGS GCRVSYERHP SQYDNISGED LQSTAPIPSV PYAPFAAILP FQHGGSSAPV 540
EFVGDFTAPE STGDPEKPPP LPEKKNKHML AYMQLLEDYS EPQPSMFYQT PQNEHIYQQK 600
NKLLMEVYGF SDSFSGVDSV QELAPPPALP PKQRQLEPPA GKDGHPRDPS AVSGVPGKDS 660
RDGSERAPKS PDALESAQSE EEVDELSLID HNEIMSRLTL KQEGDDGPDV RGGSGDILLV 720
HATETDRKDL VLYCEAFLTT YRTFISPEEL IKKLQYRYEK FSPFADTFKK RVSKNTFFVL 780
VRVVDELCLV ELTEEILKLL MELVFRLVCN GELSLARVLR KNILDKVDQK KLLRCATSSQ 840
PLAARGVAAR PGTLHDFHSH EIAEQLTLLD AELFYKIEIP EVLLWAKEQN EEKSPNLTQF 900
TEHFNNMSYW VRSIIMLQEK AQDRERLLLK FIKIMKHLRK LNNFNSYLAI LSALDSAPIR 960
RLEWQKQTSE GLAEYCTLID SSSSFRAYRA ALSEVEPPCI PYLGLILQDL TFVHLGNPDY 1020
IDGKVNFSKR WQQFNILDSM RCFQQAHYDM RRNDDIINFF NDFSDHLAEE ALWELSLKIK 1080
PRNITRRKTD REEKT 1095 |
Gene Ontology | GO:0005829; C:cytosol; TAS:Reactome. GO:0005768; C:endosome; TAS:Reactome. GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW. GO:0000186; P:activation of MAPKK activity; TAS:Reactome. GO:0001568; P:blood vessel development; IEA:Compara. GO:0016337; P:cell-cell adhesion; IEA:Compara. GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome. GO:0048008; P:platelet-derived growth factor receptor signaling pathway; IEA:Compara. GO:0051056; P:regulation of small GTPase mediated signal transduction; IEA:InterPro. GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |